|
| Status |
Public on Apr 21, 2003 |
| Title |
Treatment-specific changes in gene expression discriminate in vivo drug response in human leukemia cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To elucidate the genomics of cellular responses to cancer treatment, we analyzed the expression of over 9,600 human genes in acute lymphoblastic leukemia cells before and after in vivo treatment with methotrexate and mercaptopurine given alone or in combination. Based on changes in gene expression, we identified 124 genes that accurately discriminated among the four treatments. Discriminating genes included those involved in apoptosis, mismatch repair, cell cycle control and stress response. Only 14% of genes that changed when these medications were given as single agents also changed when they were given together. These data indicate that lymphoid leukemia cells of different molecular subtypes share common pathways of genomic response to the same treatment, that changes in gene expression are treatment-specific and that gene expression can illuminate differences in cellular response to drug combinations versus single agents.
Keywords: other
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| Contributor(s) |
Cheok MH, Yang W, Pui C, Downing JR, Cheng C, Relling MV, Evans WE |
| Citation(s) |
12704389, 20981007 |
| Submission date |
Apr 21, 2003 |
| Last update date |
Nov 04, 2019 |
| Contact name |
William E Evans |
| E-mail(s) |
william.evans@stjude.org
|
| Phone |
901-495-4995
|
| Fax |
901-495-6869
|
| URL |
http://www.stjude.org/leukemia/bio_evans.htm
|
| Organization name |
St.Jude Children's Research Hospital
|
| Department |
Pharmaceutical Sciences
|
| Lab |
Evans
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
|
| Samples (120)
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| Relations |
| BioProject |
PRJNA84963 |
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