|
| Status |
Public on Dec 02, 2011 |
| Title |
Genome-wide analysis of gene expression perturbed by Pontin shRNA in MCF7 subjected to normoxic and hypoxic conditions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to investigate the role of Pontin methylation on the expression of hypoxia responsive genes across the whole genome, we performed a microarray analysis from RNAs isolated from MCF7 cells expressing either control shRNA (shNS) or Pontin shRNA(shPontin) in normoxic and hypoxic conditions.
|
| |
|
| Overall design |
MCF7 cells were transiently transfected with either non-specific shRNA or Pontin shRNA in replicates. Cells were grown for 24 hours before being exposed to either normoxic (21% Oxygen) or hypoxic condition (1% oxygen) for 6,9 hours. Cells were then lysed and RNA isolated.
|
| |
|
| Contributor(s) |
Baek SH, Hwang D, Lee JS, Kim Y, Bhin J |
| Citation(s) |
21825155 |
| Submission date |
Mar 07, 2011 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Bhin Jin-hyuk |
| E-mail(s) |
bynjh007@postech.ac.kr
|
| Organization name |
POSTECH
|
| Department |
Department of Chemical engineering
|
| Lab |
Systems biology and Medicine
|
| Street address |
Ji-gok research center, hyoja-dong, Nam-gu
|
| City |
Pohang |
| ZIP/Postal code |
790-784 |
| Country |
South Korea |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA137927 |
Less...
More...