|
| Status |
Public on Dec 02, 2011 |
| Title |
MCF7_hypoxia 6h_Pontin_shRNA_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7_hypoxia_6h_Pontin_shRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
treatment: hypoxia
time: 6h
transfection: shRNA (shPontin)
|
| Treatment protocol |
Cells were transfected with Pontin shRNA and grown in Hypoxia for 6 to 9 hours
|
| Growth protocol |
Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina hybridization protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Illumina BeadScan 500 System
|
| Description |
MCF7 cells transfected with Pontin shRNA and grown in Hypoxia 6h
|
| Data processing |
The data were normalised using quantile normalization method with beadarray version 1.10 and lumi version 1.8.3 in R/Bioconductor package.
|
| |
|
| Submission date |
Mar 07, 2011 |
| Last update date |
Dec 02, 2011 |
| Contact name |
Bhin Jin-hyuk |
| E-mail(s) |
bynjh007@postech.ac.kr
|
| Organization name |
POSTECH
|
| Department |
Department of Chemical engineering
|
| Lab |
Systems biology and Medicine
|
| Street address |
Ji-gok research center, hyoja-dong, Nam-gu
|
| City |
Pohang |
| ZIP/Postal code |
790-784 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE27813 |
Genome-wide analysis of gene expression perturbed by Pontin shRNA in MCF7 subjected to normoxic and hypoxic conditions |
|
