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Status |
Public on Dec 02, 2011 |
Title |
MCF7_hypoxia_9h_Pontin_shRNA_rep1 |
Sample type |
RNA |
|
|
Source name |
MCF7_hypoxia_9h_Pontin_shRNA
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF7 treatment: hypoxia time: 9h transfection: shRNA (shPontin)
|
Treatment protocol |
Cells were transfected with Pontin shRNA and grown in Hypoxia for 6 to 9 hours
|
Growth protocol |
Media: DMEM, 2mM L-Glutamine and 10% Fetal Bovine Serum
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
Cy3
|
Label protocol |
Standard Illumina hybridization protocol
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Illumina BeadScan 500 System
|
Description |
MCF7 cells transfected with Pontin shRNA and grown in Hypoxia 9h
|
Data processing |
The data were normalised using quantile normalization method with beadarray version 1.10 and lumi version 1.8.3 in R/Bioconductor package.
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|
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Submission date |
Mar 07, 2011 |
Last update date |
Dec 02, 2011 |
Contact name |
Bhin Jin-hyuk |
E-mail(s) |
bynjh007@postech.ac.kr
|
Organization name |
POSTECH
|
Department |
Department of Chemical engineering
|
Lab |
Systems biology and Medicine
|
Street address |
Ji-gok research center, hyoja-dong, Nam-gu
|
City |
Pohang |
ZIP/Postal code |
790-784 |
Country |
South Korea |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE27813 |
Genome-wide analysis of gene expression perturbed by Pontin shRNA in MCF7 subjected to normoxic and hypoxic conditions |
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