|
| Status |
Public on May 01, 2024 |
| Title |
NR after 2 |
| Sample type |
RNA |
| |
|
| Source name |
After treatment in the Allergen-specific immunotherapy Non-response group
|
| Organism |
Homo sapiens |
| Characteristics |
human pbmc: peripheral blood mononuclear cells
responsive or non-respnsive sit: Non-Respnsive
before and after sit: After
|
| Treatment protocol |
Peripheral blood samples were obtained from patients with AD exhibiting positive responses to SIT (R, n=3) and those who did not show improvement with SIT (Non-R, n=3). Blood samples were collected before and after administering SIT.
|
| Growth protocol |
For transcriptome analysis, peripheral blood samples were collected from patients who had received 12 months of specific immunotherapy (SIT) using an HDM allergen extract or placebo.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PBMCs were isolated through density centrifugation on a Ficoll-Paque™ plus (Amersham Biosciences). Treg cells were sorted with a FACSAria III (BD Biosciences) using the CD3+CD4+CD25high CD127low strategy
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was then fragmented and biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit
|
| |
|
| Hybridization protocol |
Approximately 5.5 μg of labeled DNA target was hybridized to the Affymetrix GeneChip Array at 45°C for 16hour
|
| Scan protocol |
Hybridized arrays were washed and stained on a GeneChip Fluidics Station 450 and scanned on a GCS3000 Scanner (Affymetrix).
|
| Description |
Peripheral blood mononuclear cell-derived CD4+CD25hiCD127low cells in SIT non-responsive (Non-R) AD patients after immunotherpy
Human PBMC for Allergen immunotherpy
|
| Data processing |
Array data export processing and analysis was performed using Affymetrix® GeneChip Command Console® Software (AGCC). R 4.1.2 (www.r-project.org) and Bioinformatics tool (www.bioinformatics.com) were employed for all data analysis and visualization of differentially expressed genes, and the comparative analysis
log2 FC
|
| |
|
| Submission date |
Dec 28, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Kelun Zhang |
| E-mail(s) |
zhangkelun@hotmail.com
|
| Phone |
01088069381
|
| Organization name |
Yonsei university
|
| Department |
Dermatology
|
| Lab |
Skin immunology Lab
|
| Street address |
50-1, Yonsei-Ro, Seodaemun-gu
|
| City |
Seoul |
| State/province |
Seoul |
| ZIP/Postal code |
03722 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE252205 |
RORγt -expressing FOXP3+ skin-resident Treg cells derived from the periphery in Allergen-Specific Immunotherapy |
|
