|
| Status |
Public on May 10, 2024 |
| Title |
Day9 mixture, without cMS, rep2 |
| Sample type |
SRA |
| |
|
| Source name |
derived from H9 ES cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: derived from H9 ES cells
genotype: WT
treatment: Hepatic and endothelial differentiation
group: Day9 mixture, without cMS
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini plus kit (Qiagen).
NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® and NEBNext Poly(A) mRNA Magnetic Isolation Module were used as library prep kit. Total PCR cycle were 6. For RNA-seq analysis
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
replicate2
|
| Data processing |
Human reference genome (GRCh38.p13.genome.fa)
the number of reads mapped to each gene and transcripts was counted with HISAT2 and featureCounts package with GENCODE (gencode.v36.annotation.gtf)
Supplementary files format and content: tab-delimited text files include readcounts for each Sample
|
| |
|
| Submission date |
May 09, 2023 |
| Last update date |
May 10, 2024 |
| Contact name |
Koki Yoshimoto |
| E-mail(s) |
yoshimoto.koki.85c@st.kyoto-u.ac.jp
|
| Phone |
0757539775
|
| Organization name |
Kyoto university
|
| Department |
iCeMS
|
| Lab |
Kamei lab
|
| Street address |
Sakyoku-Yoshida-Ushinomiyac-ho1
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
6068302 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE232118 |
Cyclic stretching stimuli promotes HGF and MMP secretion from hESC-derived hepatoblasts and endothelial cells towards liver lobule formation in vitro. |
|
| Relations |
| BioSample |
SAMN35017695 |
| SRA |
SRX20274681 |
