|
Status |
Public on May 01, 2024 |
Title |
AD+OB_3 |
Sample type |
SRA |
|
|
Source name |
Skin
|
Organism |
Mus musculus |
Characteristics |
tissue: Skin genotype: WT treatment: AD+OB
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen). 500 ng of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using LEXOGEN Quant-Seq Library Prep Kit (Cat#001.24) standard protocols.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
Illumina Casava1.8 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence(using fastx_trimmer), then mapped to mm10 (UCSC) whole genome using Bowtie2 Read count extraction using bedtools. Assembly: mm10 Supplementary files format and content: tab-delimited text files include read count values for each Sample
|
|
|
Submission date |
Mar 09, 2023 |
Last update date |
May 01, 2024 |
Contact name |
Jeeyoun jung |
E-mail(s) |
zzen0918@gmail.com
|
Organization name |
Korea institute of Oriental Medicine
|
Street address |
Yuseong daero 1672
|
City |
DAEJEON |
ZIP/Postal code |
34054 |
Country |
South Korea |
|
|
Platform ID |
GPL19057 |
Series (1) |
GSE227042 |
Multi-omics analysis reveals altered lipid metabolism in the skin of high fat diet-fed mice of atopic dermatitis |
|
Relations |
BioSample |
SAMN33705544 |
SRA |
SRX19624228 |