|
| Status |
Public on May 01, 2024 |
| Title |
AD_5 |
| Sample type |
SRA |
| |
|
| Source name |
Skin
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Skin
genotype: WT
treatment: AD
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen). 500 ng of total RNA was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using LEXOGEN Quant-Seq Library Prep Kit (Cat#001.24) standard protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence(using fastx_trimmer), then mapped to mm10 (UCSC) whole genome using Bowtie2
Read count extraction using bedtools.
Assembly: mm10
Supplementary files format and content: tab-delimited text files include read count values for each Sample
|
| |
|
| Submission date |
Mar 09, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Jeeyoun jung |
| E-mail(s) |
zzen0918@gmail.com
|
| Organization name |
Korea institute of Oriental Medicine
|
| Street address |
Yuseong daero 1672
|
| City |
DAEJEON |
| ZIP/Postal code |
34054 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE227042 |
Multi-omics analysis reveals altered lipid metabolism in the skin of high fat diet-fed mice of atopic dermatitis |
|
| Relations |
| BioSample |
SAMN33705547 |
| SRA |
SRX19624225 |
