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Status |
Public on May 03, 2024 |
Title |
A set of circulating microRNAs belonging to the 14q32 chromosomic locus identifies two different subgroups of individuals with recent onset Stage 3 type 1 diabetes [second cohort, untargeted] |
Organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
Circulating microRNAs are linked to the onset and progression of type 1 diabetes mellitus (T1DM), making them potential biomarkers for the disease. In this study, we employ a multiplatform sequencing approach to analyze circulating microRNAs in an extended cohort of individuals with T1DM from the INNODIA consortium. Our findings reveal that a set of microRNAs located within the T1DM susceptibility chromosomal locus 14q32 distinguishes two subgroups of individuals. To validate our results, we conduct additional analyses on a second cohort of T1DM individuals, confirming the identification of these subgroups, which we have named Cluster A and Cluster B. Remarkably, Cluster B T1DM individuals, who exhibit increased expression of a set of 14q32 miRNAs, show better glycaemic control and display a different blood immunomics profile. Our findings suggest that this set of circulating microRNAs can identify two different T1DM subgroups with distinct blood immunomics at baseline and clinical outcomes during follow-up.
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Overall design |
The study design involved the analysis of two cohorts of Type 1 Diabetes (T1DM) subjects: an initial screening cohort, the INNODIA first cohort, consisting of n=115 T1DM individuals, and a validation cohort, the INNODIA second cohort, consisting of n=147 T1DM individuals. All subjects were followed-up with programmed visits at 3 (visit 2), 6 (visit 3) and 12 months (visit 4) after clinical diagnosis of T1DM. In both cohorts, blood samples were collected at baseline (visit 1) and processed within 2 hours from blood draw. The collected blood samples underwent centrifugation to separate plasma from contaminant cells and platelets. The plasma samples were then aliquoted (200 μL) and stored at -80°C in a centralized biobank. For the INNODIA first cohort, the plasma samples were subjected to miRNA profiling using two different sequencing platforms: HTG-miRNA Edge Seq and Small RNA-seq. The profiling was followed by validation using droplet digital PCR. For the validation cohort, the plasma samples were analyzed using small RNA-seq, and subsequently validated using droplet digital PCR.
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Citation missing |
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Submission date |
Apr 26, 2024 |
Last update date |
May 03, 2024 |
Contact name |
Francesco Dotta |
E-mail(s) |
francesco.dotta@unisi.it
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Organization name |
University of Siena
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Department |
Department of Medicine, surgery and neuroscience
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Street address |
Viale Bracci 16
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City |
Siena |
State/province |
Si |
ZIP/Postal code |
53100 |
Country |
Italy |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (154)
|
GSM8238847 |
plasma, T1D, S92 |
GSM8238848 |
plasma, T1D, S93 |
GSM8238849 |
plasma, T1D, S94 |
GSM8238850 |
plasma, T1D, S95 |
GSM8238851 |
plasma, T1D, S96 |
GSM8238852 |
plasma, T1D, S97 |
GSM8238853 |
plasma, T1D, S98 |
GSM8238854 |
plasma, T1D, S99 |
GSM8238855 |
plasma, T1D, S9 |
GSM8238856 |
plasma, T1D, S100 |
GSM8238857 |
plasma, T1D, S101 |
GSM8238858 |
plasma, T1D, S102 |
GSM8238859 |
plasma, T1D, S103 |
GSM8238860 |
plasma, T1D, S104 |
GSM8238861 |
plasma, T1D, S105 |
GSM8238862 |
plasma, T1D, S106 |
GSM8238863 |
plasma, T1D, S107 |
GSM8238864 |
plasma, T1D, S108 |
GSM8238865 |
plasma, T1D, S109 |
GSM8238866 |
plasma, T1D, S110 |
GSM8238867 |
plasma, T1D, S111 |
GSM8238868 |
plasma, T1D, S112 |
GSM8238869 |
plasma, T1D, S113 |
GSM8238870 |
plasma, T1D, S114 |
GSM8238871 |
plasma, T1D, S115 |
GSM8238872 |
plasma, T1D, S116 |
GSM8238873 |
plasma, T1D, S117 |
GSM8238874 |
plasma, T1D, S118 |
GSM8238875 |
plasma, T1D, S10 |
GSM8238876 |
plasma, T1D, S119 |
GSM8238877 |
plasma, T1D, S120 |
GSM8238878 |
plasma, T1D, S121 |
GSM8238879 |
plasma, T1D, S122 |
GSM8238880 |
plasma, T1D, S123 |
GSM8238881 |
plasma, T1D, S124 |
GSM8238882 |
plasma, T1D, S125 |
GSM8238883 |
plasma, T1D, S126 |
GSM8238884 |
plasma, T1D, S127 |
GSM8238885 |
plasma, T1D, S128 |
GSM8238886 |
plasma, T1D, S11 |
GSM8238887 |
plasma, T1D, S129 |
GSM8238888 |
plasma, T1D, S130 |
GSM8238889 |
plasma, T1D, S131 |
GSM8238890 |
plasma, T1D, S132 |
GSM8238891 |
plasma, T1D, S133 |
GSM8238892 |
plasma, T1D, S134 |
GSM8238893 |
plasma, T1D, S135 |
GSM8238894 |
plasma, T1D, S12 |
GSM8238895 |
plasma, T1D, S136 |
GSM8238896 |
plasma, T1D, S137 |
GSM8238897 |
plasma, T1D, S138 |
GSM8238898 |
plasma, T1D, S139 |
GSM8238899 |
plasma, T1D, S140 |
GSM8238900 |
plasma, T1D, S141 |
GSM8238901 |
plasma, T1D, S142 |
GSM8238902 |
plasma, T1D, S143 |
GSM8238903 |
plasma, T1D, S144 |
GSM8238904 |
plasma, T1D, S145 |
GSM8238905 |
plasma, T1D, S13 |
GSM8238906 |
plasma, T1D, S146 |
GSM8238907 |
plasma, T1D, S147 |
GSM8238908 |
plasma, T1D, S12b |
GSM8238909 |
plasma, T1D, S36 |
GSM8238910 |
plasma, T1D, S45 |
GSM8238911 |
plasma, T1D, S86 |
GSM8238912 |
plasma, T1D, S121b |
GSM8238913 |
plasma, T1D, S130b |
GSM8238914 |
plasma, T1D, S145b |
GSM8238915 |
plasma, T1D, S14 |
GSM8238916 |
plasma, T1D, S16 |
GSM8238917 |
plasma, T1D, S52 |
GSM8238918 |
plasma, T1D, S15 |
GSM8238919 |
plasma, T1D, S1 |
GSM8238920 |
plasma, T1D, S17 |
GSM8238921 |
plasma, T1D, S18 |
GSM8238922 |
plasma, T1D, S19 |
GSM8238923 |
plasma, T1D, S20 |
GSM8238924 |
plasma, T1D, S21 |
GSM8238925 |
plasma, T1D, S22 |
GSM8238926 |
plasma, T1D, S23 |
GSM8238927 |
plasma, T1D, S24 |
GSM8238928 |
plasma, T1D, S25 |
GSM8238929 |
plasma, T1D, S26 |
GSM8238930 |
plasma, T1D, S27 |
GSM8238931 |
plasma, T1D, S28 |
GSM8238932 |
plasma, T1D, S29 |
GSM8238933 |
plasma, T1D, S30 |
GSM8238934 |
plasma, T1D, S31 |
GSM8238935 |
plasma, T1D, S32 |
GSM8238936 |
plasma, T1D, S33 |
GSM8238937 |
plasma, T1D, S34 |
GSM8238938 |
plasma, T1D, S35 |
GSM8238939 |
plasma, T1D, S2 |
GSM8238940 |
plasma, T1D, S36b |
GSM8238941 |
plasma, T1D, S37 |
GSM8238942 |
plasma, T1D, S38 |
GSM8238943 |
plasma, T1D, S39 |
GSM8238944 |
plasma, T1D, S40 |
GSM8238945 |
plasma, T1D, S41 |
GSM8238946 |
plasma, T1D, S42 |
GSM8238947 |
plasma, T1D, S3 |
GSM8238948 |
plasma, T1D, S43 |
GSM8238949 |
plasma, T1D, S44 |
GSM8238950 |
plasma, T1D, S45b |
GSM8238951 |
plasma, T1D, S46 |
GSM8238952 |
plasma, T1D, S47 |
GSM8238953 |
plasma, T1D, S48 |
GSM8238954 |
plasma, T1D, S49 |
GSM8238955 |
plasma, T1D, S4 |
GSM8238956 |
plasma, T1D, S50 |
GSM8238957 |
plasma, T1D, S51 |
GSM8238958 |
plasma, T1D, S53 |
GSM8238959 |
plasma, T1D, S54 |
GSM8238960 |
plasma, T1D, S55 |
GSM8238961 |
plasma, T1D, S56 |
GSM8238962 |
plasma, T1D, S57 |
GSM8238963 |
plasma, T1D, S58 |
GSM8238964 |
plasma, T1D, S59 |
GSM8238965 |
plasma, T1D, S5 |
GSM8238966 |
plasma, T1D, S60 |
GSM8238967 |
plasma, T1D, S61 |
GSM8238968 |
plasma, T1D, S62 |
GSM8238969 |
plasma, T1D, S63 |
GSM8238970 |
plasma, T1D, S64 |
GSM8238971 |
plasma, T1D, S65 |
GSM8238972 |
plasma, T1D, S66 |
GSM8238973 |
plasma, T1D, S67 |
GSM8238974 |
plasma, T1D, S68 |
GSM8238975 |
plasma, T1D, S69 |
GSM8238976 |
plasma, T1D, S6 |
GSM8238977 |
plasma, T1D, S70 |
GSM8238978 |
plasma, T1D, S71 |
GSM8238979 |
plasma, T1D, S72 |
GSM8238980 |
plasma, T1D, S73 |
GSM8238981 |
plasma, T1D, S74 |
GSM8238982 |
plasma, T1D, S75 |
GSM8238983 |
plasma, T1D, S76 |
GSM8238984 |
plasma, T1D, S77 |
GSM8238985 |
plasma, T1D, S78 |
GSM8238986 |
plasma, T1D, S79 |
GSM8238987 |
plasma, T1D, S7 |
GSM8238988 |
plasma, T1D, S80 |
GSM8238989 |
plasma, T1D, S81 |
GSM8238990 |
plasma, T1D, S82 |
GSM8238991 |
plasma, T1D, S83 |
GSM8238992 |
plasma, T1D, S84 |
GSM8238993 |
plasma, T1D, S85 |
GSM8238994 |
plasma, T1D, S86b |
GSM8238995 |
plasma, T1D, S87 |
GSM8238996 |
plasma, T1D, S88 |
GSM8238997 |
plasma, T1D, S89 |
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Relations |
BioProject |
PRJNA1105084 |
Supplementary file |
Size |
Download |
File type/resource |
GSE265981_Second_cohort_untargeted.csv.gz |
492.2 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
Raw data are available in SRA |
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