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Status |
Public on May 03, 2024 |
Title |
A set of circulating microRNAs belonging to the 14q32 chromosomic locus identifies two different subgroups of individuals with recent onset Stage 3 type 1 diabetes [first cohort, targeted] |
Organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
Circulating microRNAs are linked to the onset and progression of type 1 diabetes mellitus (T1DM), making them potential biomarkers for the disease. In this study, we employ a multiplatform sequencing approach to analyze circulating microRNAs in an extended cohort of individuals with T1DM from the INNODIA consortium. Our findings reveal that a set of microRNAs located within the T1DM susceptibility chromosomal locus 14q32 distinguishes two subgroups of individuals. To validate our results, we conduct additional analyses on a second cohort of T1DM individuals, confirming the identification of these subgroups, which we have named Cluster A and Cluster B. Remarkably, Cluster B T1DM individuals, who exhibit increased expression of a set of 14q32 miRNAs, show better glycaemic control and display a different blood immunomics profile. Our findings suggest that this set of circulating microRNAs can identify two different T1DM subgroups with distinct blood immunomics at baseline and clinical outcomes during follow-up.
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Overall design |
The study design involved the analysis of two cohorts of Type 1 Diabetes (T1DM) subjects: an initial screening cohort, the INNODIA first cohort, consisting of n=115 T1DM individuals, and a validation cohort, the INNODIA second cohort, consisting of n=147 T1DM individuals. All subjects were followed-up with programmed visits at 3 (visit 2), 6 (visit 3) and 12 months (visit 4) after clinical diagnosis of T1DM. In both cohorts, blood samples were collected at baseline (visit 1) and processed within 2 hours from blood draw. The collected blood samples underwent centrifugation to separate plasma from contaminant cells and platelets. The plasma samples were then aliquoted (200 μL) and stored at -80°C in a centralized biobank. For the INNODIA first cohort, the plasma samples were subjected to miRNA profiling using two different sequencing platforms: HTG-miRNA Edge Seq and Small RNA-seq. The profiling was followed by validation using droplet digital PCR. For the validation cohort, the plasma samples were analyzed using small RNA-seq, and subsequently validated using droplet digital PCR.
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Citation missing |
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Submission date |
Apr 26, 2024 |
Last update date |
May 03, 2024 |
Contact name |
Francesco Dotta |
E-mail(s) |
francesco.dotta@unisi.it
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Organization name |
University of Siena
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Department |
Department of Medicine, surgery and neuroscience
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Street address |
Viale Bracci 16
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City |
Siena |
State/province |
Si |
ZIP/Postal code |
53100 |
Country |
Italy |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (119)
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GSM8238577 |
Plasma,T1D,S56 |
GSM8238578 |
Plasma,T1D,S57 |
GSM8238579 |
Plasma,T1D,S58 |
GSM8238580 |
Plasma,T1D,S59 |
GSM8238581 |
Plasma,T1D,S98 |
GSM8238582 |
Plasma,T1D,S99 |
GSM8238583 |
Plasma,T1D,S25 |
GSM8238584 |
Plasma,T1D,S54b |
GSM8238585 |
Plasma,T1D,S67 |
GSM8238586 |
Plasma,T1D,S68 |
GSM8238587 |
Plasma,T1D,S49 |
GSM8238588 |
Plasma,T1D,S1 |
GSM8238589 |
Plasma,T1D,S80 |
GSM8238590 |
Plasma,T1D,S60 |
GSM8238591 |
Plasma,T1D,S30 |
GSM8238592 |
Plasma,T1D,S61 |
GSM8238593 |
Plasma,T1D,S62 |
GSM8238594 |
Plasma,T1D,S63 |
GSM8238595 |
Plasma,T1D,S64 |
GSM8238596 |
Plasma,T1D,S65 |
GSM8238597 |
Plasma,T1D,S66 |
GSM8238598 |
Plasma,T1D,S35 |
GSM8238599 |
Plasma,T1D,S39 |
GSM8238600 |
Plasma,T1D,S75 |
GSM8238601 |
Plasma,T1D,S46 |
GSM8238602 |
Plasma,T1D,S77 |
GSM8238603 |
Plasma,T1D,S18 |
GSM8238604 |
Plasma,T1D,S45 |
GSM8238605 |
Plasma,T1D,S101 |
GSM8238606 |
Plasma,T1D,S94 |
GSM8238607 |
Plasma,T1D,S28 |
GSM8238608 |
Plasma,T1D,S96 |
GSM8238609 |
Plasma,T1D,S97 |
GSM8238610 |
Plasma,T1D,S93 |
GSM8238611 |
Plasma,T1D,S95 |
GSM8238612 |
Plasma,T1D,S29 |
GSM8238613 |
Plasma,T1D,S100 |
GSM8238614 |
Plasma,T1D,S85 |
GSM8238615 |
Plasma,T1D,S9 |
GSM8238616 |
Plasma,T1D,S11 |
GSM8238617 |
Plasma,T1D,S76 |
GSM8238618 |
Plasma,T1D,S53 |
GSM8238619 |
Plasma,T1D,S111 |
GSM8238620 |
Plasma,T1D,S112 |
GSM8238621 |
Plasma,T1D,S103 |
GSM8238622 |
Plasma,T1D,S104 |
GSM8238623 |
Plasma,T1D,S40 |
GSM8238624 |
Plasma,T1D,S41 |
GSM8238625 |
Plasma,T1D,S38 |
GSM8238626 |
Plasma,T1D,S24 |
GSM8238627 |
Plasma,T1D,S82 |
GSM8238628 |
Plasma,T1D,S44 |
GSM8238629 |
Plasma,T1D,S51 |
GSM8238630 |
Plasma,T1D,S52 |
GSM8238631 |
Plasma,T1D,S3 |
GSM8238632 |
Plasma,T1D,S50 |
GSM8238633 |
Plasma,T1D,S102 |
GSM8238634 |
Plasma,T1D,S107 |
GSM8238635 |
Plasma,T1D,S108 |
GSM8238636 |
Plasma,T1D,S92 |
GSM8238637 |
Plasma,T1D,S105 |
GSM8238638 |
Plasma,T1D,S6 |
GSM8238639 |
Plasma,T1D,S4 |
GSM8238640 |
Plasma,T1D,S5 |
GSM8238641 |
Plasma,T1D,S6b |
GSM8238642 |
Plasma,T1D,S7 |
GSM8238643 |
Plasma,T1D,S8 |
GSM8238644 |
Plasma,T1D,S42 |
GSM8238645 |
Plasma,T1D,S43 |
GSM8238646 |
Plasma,T1D,S69 |
GSM8238647 |
Plasma,T1D,S70 |
GSM8238648 |
Plasma,T1D,S71 |
GSM8238649 |
Plasma,T1D,S72 |
GSM8238650 |
Plasma,T1D,S74 |
GSM8238651 |
Plasma,T1D,S73 |
GSM8238652 |
Plasma,T1D,S79 |
GSM8238653 |
Plasma,T1D,S106 |
GSM8238654 |
Plasma,T1D,S106b |
GSM8238655 |
Plasma,T1D,S27 |
GSM8238656 |
Plasma,T1D,S87 |
GSM8238657 |
Plasma,T1D,S14 |
GSM8238658 |
Plasma,T1D,S88 |
GSM8238659 |
Plasma,T1D,S16 |
GSM8238660 |
Plasma,T1D,S37 |
GSM8238661 |
Plasma,T1D,S34 |
GSM8238662 |
Plasma,T1D,S36 |
GSM8238663 |
Plasma,T1D,S47 |
GSM8238664 |
Plasma,T1D,S33 |
GSM8238665 |
Plasma,T1D,S90 |
GSM8238666 |
Plasma,T1D,S89 |
GSM8238667 |
Plasma,T1D,S31 |
GSM8238668 |
Plasma,T1D,S113 |
GSM8238669 |
Plasma,T1D,S82b |
GSM8238670 |
Plasma,T1D,S78 |
GSM8238671 |
Plasma,T1D,S15 |
GSM8238672 |
Plasma,T1D,S2 |
GSM8238673 |
Plasma,T1D,S13 |
GSM8238674 |
Plasma,T1D,S114 |
GSM8238675 |
Plasma,T1D,S17 |
GSM8238676 |
Plasma,T1D,S10 |
GSM8238677 |
Plasma,T1D,S12 |
GSM8238678 |
Plasma,T1D,S19 |
GSM8238679 |
Plasma,T1D,S20 |
GSM8238680 |
Plasma,T1D,S21 |
GSM8238681 |
Plasma,T1D,S22 |
GSM8238682 |
Plasma,T1D,S23 |
GSM8238683 |
Plasma,T1D,S26 |
GSM8238684 |
Plasma,T1D,S48 |
GSM8238685 |
Plasma,T1D,S81 |
GSM8238686 |
Plasma,T1D,S83 |
GSM8238687 |
Plasma,T1D,S84 |
GSM8238688 |
Plasma,T1D,S86 |
GSM8238689 |
Plasma,T1D,S91 |
GSM8238690 |
Plasma,T1D,S109 |
GSM8238691 |
Plasma,T1D,S110 |
GSM8238692 |
Plasma,T1D,S81b |
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Relations |
BioProject |
PRJNA1105079 |
Supplementary file |
Size |
Download |
File type/resource |
GSE265976_First_cohort_Targeted.csv.gz |
406.3 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
Raw data are available in SRA |
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