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Items: 6

1.

Transcriptional analyses of two mutants of Podospora anserina impaired for sexual development

(Submitter supplied) A whole genome microarray approach has been engaged in the heterothallic euascomycete Podospora anserina to identify genes that are differentially expressed beetwen a wild type kinetics of sexual reproduction and two mutant strains ∆RID and ∆SMR1.
Organism:
Podospora anserina
Type:
Expression profiling by array
Platform:
GPL10116
17 Samples
Download data: GPR
Series
Accession:
GSE104632
ID:
200104632
2.

A genetic and functional investigation of the Zn2Cys6 transcription factors RSE2 and RSE3 in Podospora anserina

(Submitter supplied) Abstract In Podospora anserina, the two zinc cluster proteins RSE2 and RSE3 are essential for the expression of the gene encoding the alternative oxidase (aox) when the mitochondrial electron transport chain is impaired. In parallel they activated the expression of gluconeogenic genes encoding phosphoenolpyruvate carboxykinase (pck) and fructose-1,6-biphosphatase (fbp). Orthologues of these transcription factors are present in a wide range of filamentous fungi and no other role than the regulation of these three genes has been evidenced so far. more...
Organism:
Podospora anserina
Type:
Expression profiling by array
Platform:
GPL10116
28 Samples
Download data: TXT
Series
Accession:
GSE51360
ID:
200051360
3.

The transcriptional profilling of the thermosensitive self-incompatible het-R het-V strain undergoing cell death by incompatibility

(Submitter supplied) In the filamentous fungus Podospora anserina, cell death by incompatibility can be monitored using the conditional self-incompatible (SI) het-R het-V strain. SI strains are homokaryotic strains bearing incompatible het genes in all nuclei. The co-expression of these het genes triggers cell death in all the cells and hence in the entire mycelium. The het-R het-V SI strain, bearing the two incompatible het-R and het-V genes, proved particularly convenient as cell death triggering is thermosensitive (J. more...
Organism:
Podospora anserina
Type:
Expression profiling by array
Platform:
GPL10116
48 Samples
Download data: TXT
Series
Accession:
GSE21659
ID:
200021659
4.

The transcriptional response to the inactivation of the PaMpk1 and PaMpk2 MAP kinase pathways in Podospora anserina

(Submitter supplied) To gain insight into the targets of the PaMpk1 and PaMpk2 MAPK, we here determine the transcription profile of the mutants inactivated for PaMpk1, PaMpk2 and PaNox1 during vegetative growth at stages where they are required for proper development, in comparison with wild type. Microarrays representing 10556 coding sequences (CDS) predicted by the P. anserina genome project were used to estimate the transcription profiles (transcriptomes) during vegetative growth of three-day-old homokaryotic cultures of ∆PaMpk1, ∆PaMpk2 and the IDC343 PaNox1 mutants in comparison to wild type. more...
Organism:
Podospora anserina
Type:
Expression profiling by array
Platform:
GPL10116
48 Samples
Download data: TXT
Series
Accession:
GSE21331
ID:
200021331
5.

Genome-wide gene expression profiling of fertilization competent mycelium in opposite mating types in the heterothallic fungus Podospora anserina.

(Submitter supplied) Abstract BACKGROUND: Mating-type loci in yeasts and ascomycotan filamentous fungi (Pezizomycotina) encode master transcriptional factors that play a critical role in sexual development. Genome-wide analyses of mating-type-specification circuits and mating-type target genes are available in Saccharomyces cerevisiae and Schizosaccharomyces pombe; however, no such analyses have been performed in heterothallic (self-incompatible) Pezizomycotina. more...
Organism:
Podospora anserina
Type:
Expression profiling by array
Platform:
GPL10116
16 Samples
Download data: TXT
Series
Accession:
GSE27297
ID:
200027297
6.

A general framework for optimization of probes for gene expression microarray and its application to the fungus Podospora anserina

(Submitter supplied) A genome wide microarray, covering the 10546 presently known and predicted CDS, has been constructed with the Agilent in-situ synthesized 60-mers technology. 1) A library of 10 oligonucleotides per CDS has been design by the manufacturer. 2) A computational step has been performed to determine 3' position, intron position and cross-hybridization rate of all probes. These data have been compiled to attribute a score to each probe and thus, to select a first set of 4 oligonucleotides per CDS. more...
Organism:
Podospora anserina
Type:
Expression profiling by array; Genome variation profiling by array
Platforms:
GPL10115 GPL10116
27 Samples
Download data: TXT
Series
Accession:
GSE20734
ID:
200020734
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