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| Status |
Public on May 03, 2024 |
| Title |
Functional multi-organelle units control inflammatory lipid metabolism of macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Eukaryotic cells contain several membrane-separated organelles to compartmentalize distinct metabolic reactions. However, it has remained unclear how these organelle systems are coordinated, when cells adapt metabolic pathways to support their development, survival or effector functions. Here we present OrgaPlexing, a multispectral organelle imaging approach for the comprehensive mapping of six key metabolic organelles and their interactions. We use this analysis on macrophages, immune cells that undergo rapid metabolic switches upon sensing bacterial and inflammatory stimuli. Our results identify lipid droplets (LDs) as primary inflammatory responder organelle, which forms three- and four-way interactions with other organelles. While clusters with endoplasmic reticulum (ER) and mitochondria (M-ER-LD unit) help supply fatty acids for LD growth, the additional recruitment of peroxisomes (M-ER-P-LD unit) supports fatty acid efflux from LDs. Interference with individual components of these units has direct functional consequences for inflammatory lipid synthesis. Together, we show that macrophages form functional multi-organellar units (MOUs) to support metabolic adaptation, and provide an experimental strategy to identify organelle-metabolic signaling hubs.
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| Overall design |
bone marrow-derived mouse macrophages treated with LPS/IFNg for 0, 2, 6 and 24 hours
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| Contributor(s) |
Zimmermann J, Deboutte W, Manke T, Bremser A, Rambold A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 21, 2023 |
| Last update date |
May 03, 2024 |
| Contact name |
Angelika Rambold |
| E-mail(s) |
ramboldlab@gmail.com
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| Organization name |
Max Planck Institute of Immunobiology and Epigenetics
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| Department |
Department of Developmental Immunology
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| Street address |
Stübeweg 51
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| City |
Freiburg im Breisgau |
| ZIP/Postal code |
79108 |
| Country |
Germany |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (12)
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| GSM7503622 |
BMDM, untreated, 0 hours, rep4 |
| GSM7503623 |
BMDM, untreated, 0 hours, rep5 |
| GSM7503624 |
BMDM, treated, 2 hours, rep1 |
| GSM7503625 |
BMDM, treated, 2 hours, rep2 |
| GSM7503626 |
BMDM, treated, 6 hours, rep1 |
| GSM7503627 |
BMDM, treated, 6 hours, rep2 |
| GSM7503628 |
BMDM, treated, 24 hours, rep1 |
| GSM7503629 |
BMDM, treated, 24 hours, rep2 |
| GSM7503630 |
BMDM, treated, 24 hours, rep3 |
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| Relations |
| BioProject |
PRJNA986140 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE235484_counts.tsv.gz |
530.0 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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