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    TAG1 membrane bound O-acyl transferase (MBOAT) family protein [ Arabidopsis thaliana (thale cress) ]

    Gene ID: 816464, updated on 7-Sep-2023

    Summary

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    Gene symbol
    TAG1
    Gene description
    membrane bound O-acyl transferase (MBOAT) family protein
    Primary source
    Araport:AT2G19450
    Locus tag
    AT2G19450
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Arabidopsis thaliana (ecotype: Columbia)
    Lineage
    Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
    Also known as
    ABX45; acyl-CoA:diacylglycerol acyltransferase 1; Arabidopsis thaliana acyl-CoA:diacylglycerol acyltransferase; AS11; ATDGAT; AtDGAT1; DGAT1; DIACYLGLYCEROL ACYLTRANSFERASE; F27F23.24; RDS1; TRIACYLGLYCEROL BIOSYNTHESIS DEFECT 1
    Summary
    Encodes Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the final step of the triacylglycerol synthesis pathway. An insertion mutation in the TAG1 gene results in altered lipid phenotype. Role in senescence and seed development. Its preferred substrate is linolenoyl-CoA (C18:3-CoA).
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    Genomic context

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    See TAG1 in Genome Data Viewer
    Location:
    chromosome: 2
    Exon count:
    16
    Sequence:
    Chromosome: 2; NC_003071.7 (8426117..8429853)

    Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene DWD (DDB1-binding WD40 protein) hypersensitive to ABA 1 Neighboring gene O-Glycosyl hydrolases family 17 protein Neighboring gene DUF3511 domain protein (DUF3511) Neighboring gene miscRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003071.7

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. In vivo packaging of triacylglycerols enhances Arabidopsis leaf biomass and energy density. Winichayakul S, et al. Plant Physiol, 2013 Jun. PMID 23616604, Free PMC Article
    2. A role for diacylglycerol acyltransferase during leaf senescence. Kaup MT, et al. Plant Physiol, 2002 Aug. PMID 12177474, Free PMC Article
    3. Arabidopsis mutants deficient in diacylglycerol acyltransferase display increased sensitivity to abscisic acid, sugars, and osmotic stress during germination and seedling development. Lu C, et al. Plant Physiol, 2002 Jul. PMID 12114588, Free PMC Article
    4. Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Jako C, et al. Plant Physiol, 2001 Jun. PMID 11402213, Free PMC Article
    5. The TAG1 locus of Arabidopsis encodes for a diacylglycerol acyltransferase. Routaboul JM, et al. Plant Physiol Biochem, 1999 Nov. PMID 10580283

    See all (25) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. On the Role of DGAT1 in Seed Glycerolipid Metabolic Network and Critical Stages of Plant Development in Arabidopsis.
      Title: On the Role of DGAT1 in Seed Glycerolipid Metabolic Network and Critical Stages of Plant Development in Arabidopsis.
    2. Oil-Producing Metabolons Containing DGAT1 Use Separate Substrate Pools from those Containing DGAT2 or PDAT.
      Title: Oil-Producing Metabolons Containing DGAT1 Use Separate Substrate Pools from those Containing DGAT2 or PDAT.
    3. The conversion of diacylglycerol to triacylglycerol by DGAT1 is critical for plant freezing tolerance.
      Title: DIACYLGLYCEROL ACYLTRANSFERASE and DIACYLGLYCEROL KINASE Modulate Triacylglycerol and Phosphatidic Acid Production in the Plant Response to Freezing Stress.
    4. DGAT1 expression is regulated by MYB96 in the Arabidopsis seeds.
      Title: The MYB96 Transcription Factor Regulates Triacylglycerol Accumulation by Activating DGAT1 and PDAT1 Expression in Arabidopsis Seeds.
    5. The constructed promoter was ligated upstream of the TAG1 gene encoding diacylglycerol acyltransferase 1 and introduced into Arabidopsis. Seeds from transgenic plants carrying AtTAG1 under the control of the chimeric promoter showed increased oil content (up by 1873%) compared with wild-type seeds.
      Title: Design of a Seed-Specific Chimeric Promoter with a Modified Expression Profile to Improve Seed Oil Content.
    6. This study identifies the first two regulatory genes, WRI1 and DGAT1, that control the synthesis of all tocochromanol forms in seeds, and shows the existence of a metabolic trade-off between lipid and tocochromanol metabolisms.
      Title: WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis.
    7. The results suggest that dedifferentiation is associated with Tag1 activation and that CMT3 rather than DDM1 plays a central role in restraining Tag1 activation via inducing gene body DNA methylation.
      Title: Activation of Tag1 transposable elements in Arabidopsis dedifferentiating cells and their regulation by CHROMOMETHYLASE 3-mediated CHG methylation.
    8. ABI4 and ABI5 synergistically regulate DGAT1 expression in Arabidopsis seedlings under stress.
      Title: ABA-insensitive (ABI) 4 and ABI5 synergistically regulate DGAT1 expression in Arabidopsis seedlings under stress.
    9. Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2.
      Title: Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2.
    10. ABI4 is essential for the activation of DGAT1 in Arabidopsis seedlings during nitrogen deficiency.
      Title: ABI4 activates DGAT1 expression in Arabidopsis seedlings during nitrogen deficiency.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    NM_127503.3

    Gene Ontology Provided by TAIR

    Function Evidence Code Pubs
    enables diacylglycerol O-acyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables diacylglycerol O-acyltransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables diacylglycerol O-acyltransferase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within carbohydrate metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within embryo development ending in seed dormancy TAS
    Traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of seed germination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of embryonic development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of seed germination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to abscisic acid IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to cold IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to glucose IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within response to salt stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within triglyceride biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in triglyceride biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within triglyceride biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within triglyceride biosynthetic process TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in chloroplast envelope IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in lipid droplet IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane TAS
    Traceable Author Statement
    more info
    		 PubMed 
    located_in nucleus ISM
    Inferred from Sequence Model
    more info
    		  

    General protein information

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    Preferred Names
    membrane bound O-acyl transferase (MBOAT) family protein
    NP_179535.1
    • TRIACYLGLYCEROL BIOSYNTHESIS DEFECT 1 (TAG1); CONTAINS InterPro DOMAIN/s: Membrane bound O-acyl transferase, MBOAT (InterPro:IPR004299); Has 951 Blast hits to 945 proteins in 279 species: Archae - 0; Bacteria - 104; Metazoa - 315; Fungi - 305; Plants - 83; Viruses - 0; Other Eukaryotes - 144 (source: NCBI BLink).

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003071.7 Reference assembly

      Range
      8426117..8429853
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_127503.3 → NP_179535.1  membrane bound O-acyl transferase (MBOAT) family protein [Arabidopsis thaliana]

      See identical proteins and their annotated locations for NP_179535.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      Q9S7F2, Q9SLD2
      UniProtKB/TrEMBL
      A0A178VVE4, A0A5S9WZA8
      Conserved Domains (1) summary
      PLN02401
      Location:101 → 519
      PLN02401; diacylglycerol o-acyltransferase
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9SLD2.2

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