ABA-insensitive (ABI) 4 and ABI5 synergistically regulate DGAT1 expression in Arabidopsis seedlings under stress

Yinfei Kong; Songbin Chen; Yang Yang; Chengcai An

doi:10.1016/j.febslet.2013.07.045

ABA-insensitive (ABI) 4 and ABI5 synergistically regulate DGAT1 expression in Arabidopsis seedlings under stress

FEBS Lett. 2013 Sep 17;587(18):3076-82. doi: 10.1016/j.febslet.2013.07.045. Epub 2013 Aug 11.

Authors

Yinfei Kong¹, Songbin Chen, Yang Yang, Chengcai An

Affiliation

¹ State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.

PMID: 23942253
DOI: 10.1016/j.febslet.2013.07.045

Abstract

Triacylglycerol (TAG) accumulation is essential for seed maturation in plants. Diacylglycerol acyltransferase 1 (DGAT1) is the rate-limiting enzyme in TAG biosynthesis. In this study, we show that TAG accumulation in Arabidopsis seedlings is correlated with environmental stress, and both ABI4 and ABI5 play important roles in regulating DGAT1 expression. Tobacco transient assays revealed the synergistic effect of ABI4 with ABI5 in regulating DGAT1 expression. Taken together, our findings indicate ABI5 is an important accessory factor with ABI4 in the activation of DGAT1 in Arabidopsis seedlings under stress.

Keywords: ABI4; ABI5; DGAT1; Synergistic effect; Triacylglycerol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid / pharmacology
Agrobacterium tumefaciens / genetics
Agrobacterium tumefaciens / metabolism
Arabidopsis / drug effects
Arabidopsis / genetics*
Arabidopsis / metabolism
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Basic-Leucine Zipper Transcription Factors / genetics*
Basic-Leucine Zipper Transcription Factors / metabolism
Diacylglycerol O-Acyltransferase / genetics*
Diacylglycerol O-Acyltransferase / metabolism
Gene Expression Regulation, Plant*
Genes, Reporter
Germination / drug effects
Luciferases
Nicotiana / genetics
Nicotiana / metabolism
Plant Growth Regulators / pharmacology
Plants, Genetically Modified / drug effects
Plants, Genetically Modified / genetics*
Plants, Genetically Modified / metabolism
Seedlings / drug effects
Seedlings / genetics*
Seedlings / metabolism
Stress, Physiological
Transcription Factors / genetics*
Transcription Factors / metabolism
Triglycerides / biosynthesis

Substances

ABI4 protein, Arabidopsis
ABI5 protein, Arabidopsis
Arabidopsis Proteins
Basic-Leucine Zipper Transcription Factors
Plant Growth Regulators
Transcription Factors
Triglycerides
Abscisic Acid
Luciferases
DGAT1 protein, Arabidopsis
Diacylglycerol O-Acyltransferase