|
| Status |
Public on Dec 31, 2019 |
| Title |
Infected_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Caco-2 cells grown on transwells - Infected With JEV
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Caco-2
cell morphology: polarized
infection status: Japanese encephalitis virus
|
| Treatment protocol |
Infected with an MOI of 5 pfu/cell of Japanese encephalitis virus
|
| Growth protocol |
Caco-2 cells grown on transwells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA prepared using Qiagen RNA extraction kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human PrimeView Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from embryos in slow phase of cellularisation.
I2.as.rma-Signal
|
| Data processing |
The data were analyzed with Expression Console and Gene springGX 12.0 using Affymetrix default analysis settings and RMA as normalization method used. The probes of intensities less than the 20.0 percentile in the raw data were excluded.
|
| |
|
| Submission date |
Jul 30, 2012 |
| Last update date |
Dec 31, 2019 |
| Contact name |
Guruprasad Ramarao Medigeshi |
| E-mail(s) |
gmedigeshi@thsti.res.in
|
| Phone |
911292876311
|
| Organization name |
Translational Health Science and Technology Institute
|
| Department |
Vaccine and Infectious Disease Research Center
|
| Lab |
Virology
|
| Street address |
NCR Biotech Science Cluster, P.O. Box No. 4
|
| City |
Faridabad |
| State/province |
Haryana |
| ZIP/Postal code |
121001 |
| Country |
India |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE39740 |
Gene expression profile in Caco-2 cells infected with Japanese encephalitis virus |
|
