|
| Status |
Public on Jul 26, 2012 |
| Title |
+/+ heart 2 |
| Sample type |
RNA |
| |
|
| Source name |
heart muscle, transgenic
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: transgenic
tissue: heart muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared according to the Affymetrix Whole Transkript Sense Labelling protocol from100 ng total RNA according to the manufacturer
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip MoGene-1.0-st-v1 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000 gene chip scanner with GCOS 1.4.
|
| Data processing |
The data were analysed using Bioconductor package 'xps' under R-2.10.0. Background correction, normalization and probe summarization was performed with RMA for each tissue separately using the core transcript annotation.
|
| |
|
| Submission date |
Jul 25, 2012 |
| Last update date |
Jul 26, 2012 |
| Contact name |
Stephan Direnberger |
| E-mail(s) |
direnberger@bio.lmu.de
|
| Organization name |
Ludwig-Maximilians University
|
| Street address |
Grosshaderner Str. 2
|
| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE39632 |
Gene Expression profiling of transgenic mice expressing the genetically encoded calcium indicator TN-XXL in muscle and brain tissues |
|
