|
| Status |
Public on Dec 01, 2012 |
| Title |
LERKO biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Liver selective ERalpha knockout mouse
|
| Organism |
Mus musculus |
| Characteristics |
Sex: female
genotype: Liver selective ERalpha knockout mouse
tissue: liver
genetic background: C57BL/6J
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from frozen mouse tissues using the Trizol reagent (Invitrogen) as per manufacturer’s instructions. The isolated RNA was subsequently purified with the RNeasy Plus Mini Kits (Qiagen),
|
| Label |
biotin
|
| Label protocol |
Fragmented single-stranded sense DNA was end labeled with biotin according to the Affymetrx Gene chip WT Terminal Labeling and hybridization user manual
|
| |
|
| Hybridization protocol |
The Gene Chip Mouse Gene 1.1 ST Array Plate was hybridized in the Affymetrix Gene Titan Instrument standard procedure
|
| Scan protocol |
The Gene Chip Mouse Gene 1.1 ST Array Plate was scanned in Affymetrix Gene Titan Instrument standard procedure
|
| Data processing |
The data were analyzed with oilgo package (version 1.16.0) in Bioconductor. Transcripts level were summaried for core probesets using RMA.
|
| |
|
| Submission date |
Mar 14, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Marko Matic |
| Organization name |
Karolinska Institutet
|
| Street address |
Halsovägen 7
|
| City |
Huddinge |
| ZIP/Postal code |
14183 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL11533 |
| Series (1) |
| GSE36514 |
Estrogen signaling and the Metabolic Syndrome: Targeting the hepatic ERalpha action |
|
