|
| Status |
Public on Feb 24, 2013 |
| Title |
PT12 estrogen withdrawal, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Tumor
|
| Organism |
Homo sapiens |
| Characteristics |
xenograft: PT12
treatment: estrogen withdrawal
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 500ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.5 ug of cDNA were hybridized for 20 hr at 48C on HuGene 1.1ST Array strip. GeneStrips were washed and stained in the Affymetrix Gene Atlas fluidics station.
|
| Scan protocol |
GeneStrips were scanned using the Gene Atlas imaging station.
|
| Description |
Human breast cancer was xenografted in NOD/SCID mice
|
| Data processing |
The data were analyzed with Partek using RMA as normalization method.
|
| |
|
| Submission date |
Feb 24, 2012 |
| Last update date |
Feb 24, 2013 |
| Contact name |
peter kabos |
| E-mail(s) |
peter.kabos@ucdenver.edu
|
| Organization name |
University of Colorado Denver
|
| Department |
Medicine
|
| Street address |
12801 E. 17th Avenue
|
| City |
Aurora |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE36075 |
Estrogen receptor dynamics in diverse patient-derived luminal breast cancer xenografts |
|
