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Status |
Public on Apr 24, 2024 |
Title |
shRPL35A_01 |
Sample type |
RNA |
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Source name |
BC457-1_(PrimeView)
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Organism |
Homo sapiens |
Characteristics |
cell line: NCI-H1299 treatment: infected with shRPL35A
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Treatment protocol |
NCI-H1299 cells in logarithmic growth phase were infected by adding 20 μL 1×108 TU/mL shCtrl or shRPL35A lentivirus, culturing in 1640 medium with 10% FBS in a 6-well dish with 2×105 cells per well.
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Growth protocol |
NCI-H1299 cells were cultured in 1640 medium containing 10% fetal bovine serum (FBS) in cell incubator with 5% CO2 at 37°C.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
3’ IVT Biotin Label
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on GeneChip primeview human PathArrayTM.
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Scan protocol |
Affymetrix human GeneChip PrimeView and the outcomes were scanned by Affymetrix Scanner 3000.
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Data processing |
The statistical significance of raw data was completed by a Welch t-test with Benjamini-Hochberg FDR.
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Submission date |
Apr 19, 2024 |
Last update date |
Apr 24, 2024 |
Contact name |
Liqin Yan |
E-mail(s) |
syyyylq@d.sxmu.edu.cn, YLQ691450@163.com
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Phone |
+8613834691450
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Organization name |
Shanxi Medical University
|
Street address |
Jiefang Road
|
City |
Taiyuan |
ZIP/Postal code |
030000 |
Country |
China |
|
|
Platform ID |
GPL15207 |
Series (1) |
GSE264428 |
Gene expression in NCI-H1299 cells infected with shCtrl and shRPL35A |
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