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| Status |
Public on Apr 25, 2024 |
| Title |
adult pancreas; PB2032-PAAD-T_S4_L002 |
| Sample type |
SRA |
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| Source name |
pancreas
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| Organism |
Homo sapiens |
| Characteristics |
tissue: pancreas
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| Extracted molecule |
total RNA |
| Extraction protocol |
All remaining tissues are collected in MACS® Tissue Storage Solution (Miltenyi Biotec., Auburn, CA, USA) on ice, and processed to the single cell dissociation immediately.
The sequenced libraries were processed using the HiSeqX platform from Illumina to obtain the requisite data for our study.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
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| Data processing |
The Cell Ranger pipeline was applied for pre-processing, including demultiplexing of sequencing results, barcode processing, read alignment, filtering of estimated droplets, and the generation of gene/barcode matrices. Reads were aligned to the GRCh38 human reference genome, and the UMI matrix of cell barcodes according to genes was generated for each sample.
Assembly: GRCh38
Supplementary files format and content: Raw_counts.txt.gz is a tab-delimited text file which includes UMI counts for all cells.
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| Submission date |
Apr 11, 2024 |
| Last update date |
Apr 25, 2024 |
| Contact name |
Semin Lee |
| E-mail(s) |
seminlee@unist.ac.kr
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| Organization name |
Ulsan National Institute of Science and Technology
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| Department |
Biomedical Engineering
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| Street address |
50, UNIST-gil
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| City |
Ulsan |
| ZIP/Postal code |
44919 |
| Country |
South Korea |
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| Platform ID |
GPL20795 |
| Series (1) |
| GSE263733 |
Single-cell transcriptome analysis reveals subtype-specific clonal evolution and microenvironmental changes in liver metastasis of pancreatic adenocarcinoma and their clinical implications |
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| Relations |
| BioSample |
SAMN33922015 |
| SRA |
SRX19778805 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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