|
| Status |
Public on May 07, 2024 |
| Title |
N_C_mt of TAp73 biological rep3 batch 1 |
| Sample type |
RNA |
| |
|
| Source name |
lung cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H1299
transfection: N_C_mt of TAp73
|
| Treatment protocol |
Retrovirus carrying TAp73 was generated by transfecting 293T cells with pBABE empty vector or those with the respective genes, together with the helper plasmids. H1299 cells were infected with harvested retrovirus soup for 48 hrs, and were selected with 2 µg/ml puromycin for another 2 days, and used for the experiments.
|
| Growth protocol |
H1299 cells were cultured with DMEM medium with 10% FBS and 1% PenStrep
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extract by using RNA extraction Kit
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to standard Affymetrix protocols.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to standard protocols.
|
| Scan protocol |
Scanning was performed according to standard protocols.
|
| Data processing |
The R package affy was used for data processing. The rma function (Robust Multiarray Average) was used for quantile normalization and background correction.
|
| |
|
| Submission date |
Mar 22, 2024 |
| Last update date |
May 07, 2024 |
| Contact name |
Kanaga Sabapathy |
| E-mail(s) |
kanaga.sabapathy@ntu.edu.sg
|
| Organization name |
Nanyang Technological University Singapore
|
| Department |
SBS
|
| Street address |
60 Nanyang Drive
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
637551 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE262279 |
Dichotomous transactivation domains contribute to growth inhibitory and promotion functions of TAp73 |
|
