|
| Status |
Public on Apr 29, 2024 |
| Title |
microglia MGcCI 1m rep3 |
| Sample type |
RNA |
| |
|
| Source name |
FACS-isolated microglia control 1m
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Brain cortex
animal age: 1-month-old
gender: Male
strain: C57BL/6
|
| Treatment protocol |
Mice were anesthetized and transcardially perfused with PBS.
|
| Growth protocol |
Mice were housed under controlled temperature (22ºC) and humidity conditions in a 12 h light/12 h dark cycle with ad libitum access to food and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Brain cortex was dissected in HBSS (no calcium, no magnesium), and homogenized and disaggregated by physical and papain treatments. Percoll gradient centrifugation was used to separate cells from the rest of the tissue.
Cells were stained with the antibodies CD11b-BV711, CD45-PE and ACSA2-APC. Microglia cells (immunoreactive for both CD11b-BV711,CD45-PE) and astrocytes (immunoreactive for ACSA2-APC) were isolated by FACS.
RNA was extracted using TRIzol reagent.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
cDNA synthesis, hybridization and stain were performed using the Clariom S Pico Assay, mouse (Applied Biosystems).
|
| Scan protocol |
Array scanning was performed using the Expression Console Software and the Scanner 3000 (Affymetrix).
|
| Description |
Gene expression data from microglia isolated from 1-month-old MGcCI mouse brain cortex
|
| Data processing |
Raw data from were exported to the R enviroment using LIMMA/Bioconductor packages.
logFC: log2-fold change; AveExpr: average log2-expression level for that gene across all the arrays and channels in the experiment; t: the moderated t-statistic; P.Value: associated p-value; adj.P.Value: p-value adjusted for multiple testing.
|
| |
|
| Submission date |
Jan 30, 2024 |
| Last update date |
Apr 29, 2024 |
| Contact name |
Juan Jose Perez-Moreno |
| E-mail(s) |
jpmoreno@us.es
|
| Organization name |
University of Seville
|
| Department |
Cell Biology
|
| Street address |
Instituto de Biomedicina de Sevilla (IBiS), Campus Hospital Universitario Virgen del Rocío, Avda. Manuel Siurot
|
| City |
Seville |
| ZIP/Postal code |
41013 |
| Country |
Spain |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE254585 |
Expression data from mice cortical microglia and astrocytes. |
|
