|
Status |
Public on May 09, 2024 |
Title |
C57BL/6 control lung |
Sample type |
SRA |
|
|
Source name |
lung
|
Organism |
Mus musculus |
Characteristics |
tissue: lung
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Following dissociation into single cell suspension, single-cell 3’ RNA-Seq libraries were prepared using Chromium Single Cell 3′RNA-sequencing system (10x Genomics) with the Reagent Kit v3.1 (10XGenomics, PN-1000121) according to the manufacturer's instructions Libraries were constructed per 10x protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
C57BL/6 control lung
|
Data processing |
BCL files were converted to fastq files using cellranger mkfastq Fastq files were processed using cellranger count Further processing and analyses performed are outlined on GitHub (https://github.com/kidcancerlab/epithelial) Assembly: mm10 Supplementary files format and content: Tar files for each sample include the contents of the filtered_feature_bc_matrix folder output by Cellranger count (barcodes.tsv.gz features.tsv.gz matrix.mtx.gz)
|
|
|
Submission date |
Jan 08, 2024 |
Last update date |
May 09, 2024 |
Contact name |
Matt Cannon |
E-mail(s) |
matthewvc1@gmail.com
|
Organization name |
Nationwide Children's Hospital
|
Department |
Center for Childhood Cancer
|
Street address |
700 Children's Dr.
|
City |
Columbus |
State/province |
OH |
ZIP/Postal code |
43205 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE252703 |
Aberrant activation of wound healing programs within the metastatic niche facilitates lung colonization by osteosarcoma cells |
|
Relations |
BioSample |
SAMN39291023 |
SRA |
SRX23128551 |