|
| Status |
Public on May 01, 2024 |
| Title |
MM 5 LY6E- |
| Sample type |
RNA |
| |
|
| Source name |
Multiple Myeloma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: CD138+ myeloma plasma cells
status after 5 years: Live/Dead
age: 64 years
Sex: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples are collected from bone marrow aspirates of multiple myeloma patients
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Myeloma patient 5
|
| Data processing |
GCOS1.1 software (Affymetrix, Santa Clara, CA) and normalized using conventional GCOS1.1 scaling.
|
| |
|
| Submission date |
May 30, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Intawat Nookaew |
| E-mail(s) |
inookaew@uams.edu
|
| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Department of Biomedical Informatics
|
| Street address |
4301 W Markham St
|
| City |
Little Rock |
| ZIP/Postal code |
72205 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE233740 |
Role of LY6E subpopulation on myeloma cell growth |
|
