|
| Status |
Public on May 10, 2024 |
| Title |
pcPrF-M28 |
| Sample type |
RNA |
| |
|
| Source name |
primary cultured prostate fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: prostate cancer
Sex: Male
Stage: cT4N1M1ab
gleason score: 4+5
|
| Treatment protocol |
The culture medium was replaced with phenol red (-) RPMI-1640 supplemented with 1% CS-FBS containing DHT (0.1 nM) and cultured for 48 h.
|
| Growth protocol |
Fibroblasts (1.0 × 105 cells/well) were seeded in 6-well plates with SCBM Bullet Kit (Lonza Group Ltd.) and were cultured for 72 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using miRNesy mini kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Extracted total RNA was labeled with Cy5 using the 3D-Gene miRNA labeling kit (Toray, Kamakura, Japan)
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 32 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner (Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
May 11, 2023 |
| Last update date |
May 10, 2024 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL29077 |
| Series (1) |
| GSE232256 |
Fibroblast-derived exosomal microRNA regulates NKX3-1 expression in androgen-sensitive, androgen receptor-dependent prostate cancer cells |
|
