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Status |
Public on Aug 26, 2006 |
Title |
FACS-purified B cells_129SV/EV_IFN-b-stimulated_rep1 |
Sample type |
RNA |
|
|
Source name |
B cells - wildtype mice - IFN-b stimulated
|
Organism |
Mus musculus |
Characteristics |
Strain: 129SV/EV Gender: Female Age: 8-12weeks Tissue: Lymph node B cell 17h treatment IFN-beta (2000U/ml)
|
Biomaterial provider |
B&K Universal Ltd.- U. K.
|
Treatment protocol |
In vitro exposure to IFN-b (17 hours, 2000U/ml)
|
Growth protocol |
Mice were bred and held under conventional housing conditions in filtertop cages
|
Extracted molecule |
total RNA |
Extraction protocol |
RNAeasy kit (Qiagen)
|
Label |
Biotin
|
Label protocol |
Bioarray (Enzo)
|
|
|
Hybridization protocol |
Hybridized washed and stained with streptavidin-PE per the manufacturer's protocol (Affymetrix).
|
Scan protocol |
Affymetrix GeneChip Scanner 3000 and GCOS software using target value = 262
|
Description |
B cells from wild type mice treated with IFN-b Array 23 - 23.N.B._565.CEL
|
Data processing |
The dataset was preprocessed using the following procedures: (1) background correction; (2) normalization using the quantile method (Bolstad et al. 2003); (3) summarization of probe set values using the RMA (Robust Multi-array Analysis) method, which fits a specified robust linear model to the probe level data. The abovementioned procedures were performed using the Bioconductor package “affyPLM”.
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|
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Submission date |
Aug 25, 2005 |
Last update date |
Aug 28, 2018 |
Contact name |
Michael E. Salazar |
Phone |
(415) 514-4371
|
URL |
http://arrays.ucsf.edu
|
Organization name |
University of California, San Francisco
|
Department |
|
Lab |
Functional Genomics Core Laboratories
|
Street address |
1550 Fourth Street, RM 545
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94158 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE3203 |
Influenza virus infection-induced gene expression changes of regional B cells are mediated in part through type I IFN |
|
Relations |
Reanalyzed by |
GSE119085 |