|
| Status |
Public on Dec 31, 2012 |
| Title |
J82_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
J82_rep3
|
| Organism |
Homo sapiens |
| Characteristics |
sensitivity to hedgehog: Insensitive
cell line: J82
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN Rneasy mini kit. Total RNA was quantified with a Nanodrop 8000 Spectrophotometer and quality control was performed with an Agilent Bioanalyser 2100 using the Agilent RNA 6000 Nani kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep Amplification Kit.
|
| |
|
| Hybridization protocol |
Standar Illumina hybridization protocol
|
| Scan protocol |
Standar Illumina scanning protocol
|
| Description |
Replicate 3
|
| Data processing |
Quantile normalization algorithm were used to normalised the values of the probe sets within each sample. The software used for normalization was GeneSpring GX v11.0, Agilent Technologies Inc.
|
| |
|
| Submission date |
Mar 29, 2011 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Avencia Sanchez-Mejias |
| E-mail(s) |
asmejias@med.miami.edu
|
| Phone |
3057789602
|
| Organization name |
University of Miami
|
| Department |
Surgery
|
| Lab |
Robbins
|
| Street address |
1600 NW 10th Ave
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE28255 |
Gene expression analysis in bladder cancer cells lines |
|
