|
| Status |
Public on Dec 30, 2011 |
| Title |
IL-22 3 |
| Sample type |
RNA |
| |
|
| Source name |
IL-22
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: keratinocytes
cell line: HaCaT
treatment: IL-22
|
| Treatment protocol |
samples were exposed to IL-22 at 200ng/ml for 12h
|
| Growth protocol |
HaCaT cells were grown in DMEM medium (1.5mM Ca), supplemented with 10% FCS, p/s and glu
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Plus Mini Kit
|
| Label |
biotin
|
| Label protocol |
standard Illumina protocol
|
| |
|
| Hybridization protocol |
standard Illumina protocol
|
| Scan protocol |
standard Illumina protocol using Illumina iScan array scanner
|
| Description |
replicate 3
|
| Data processing |
Illumina' Genomestudio v.2010.1 with default settings
using the Bioconductor package 'lumi' lumiN and lumiT was used to normalize and transform the data
|
| |
|
| Submission date |
Mar 24, 2011 |
| Last update date |
Dec 30, 2011 |
| Contact name |
Stephen Taylor |
| E-mail(s) |
stephen.taylor@imm.ox.ac.uk
|
| Phone |
+44 1865 222640
|
| Organization name |
CBRG
|
| Street address |
Headington
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE28158 |
IL-22 down-regulates filaggrin expression and affects expression of profilaggrin processing enzymes by keratinocytes |
|
