|
Status |
Public on Dec 30, 2011 |
Title |
IL-22 2 |
Sample type |
RNA |
|
|
Source name |
IL-22
|
Organism |
Homo sapiens |
Characteristics |
cell type: keratinocytes cell line: HaCaT treatment: IL-22
|
Treatment protocol |
samples were exposed to IL-22 at 200ng/ml for 12h
|
Growth protocol |
HaCaT cells were grown in DMEM medium (1.5mM Ca), supplemented with 10% FCS, p/s and glu
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy Plus Mini Kit
|
Label |
biotin
|
Label protocol |
standard Illumina protocol
|
|
|
Hybridization protocol |
standard Illumina protocol
|
Scan protocol |
standard Illumina protocol using Illumina iScan array scanner
|
Description |
replicate 2
|
Data processing |
Illumina' Genomestudio v.2010.1 with default settings using the Bioconductor package 'lumi' lumiN and lumiT was used to normalize and transform the data
|
|
|
Submission date |
Mar 24, 2011 |
Last update date |
Dec 30, 2011 |
Contact name |
Stephen Taylor |
E-mail(s) |
stephen.taylor@imm.ox.ac.uk
|
Phone |
+44 1865 222640
|
Organization name |
CBRG
|
Street address |
Headington
|
City |
Oxford |
ZIP/Postal code |
OX3 9DS |
Country |
United Kingdom |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE28158 |
IL-22 down-regulates filaggrin expression and affects expression of profilaggrin processing enzymes by keratinocytes |
|