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Sample GSM67369

Query DataSets for GSM67369

Status

Public on Sep 26, 2005

Title

del337TR replicate 1

Sample type

RNA

Source name

Mouse heart

Organism

Mus musculus

Characteristics

deletion in 337TR

Extracted molecule

total RNA

Extraction protocol

Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

deletion in 337TR

Data processing

Robust Multichip Analysis (RMA)

Submission date

Aug 05, 2005

Last update date

Sep 26, 2005

Contact name

Norman Buroker

E-mail(s)

Norman.Buroker@seattlechildrens.org

Organization name

Seattle Children's Hospital

Lab

Portman

Street address

4800 Sand Point Way N.E.

City

Seattle

State/province

ZIP/Postal code

98105

Country

USA

Platform ID

GPL1261

Series (1)

GSE3067

Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	245.3	P	0.001102
AFFX-BioB-M_at	251.3	P	0.000225
AFFX-BioB-3_at	158.5	P	0.00141
AFFX-BioC-5_at	488.3	P	0.00011
AFFX-BioC-3_at	379.3	P	0.000052
AFFX-BioDn-5_at	584	P	0.00006
AFFX-BioDn-3_at	2959.2	P	0.000258
AFFX-CreX-5_at	6714.2	P	0.000052
AFFX-CreX-3_at	7825.4	P	0.000044
AFFX-DapX-5_at	27.5	A	0.131361
AFFX-DapX-M_at	29.4	A	0.175328
AFFX-DapX-3_at	55.3	A	0.089478
AFFX-LysX-5_at	3.3	A	0.470241
AFFX-LysX-M_at	21.8	A	0.58862
AFFX-LysX-3_at	27.4	P	0.030976
AFFX-PheX-5_at	829.9	P	0.000044
AFFX-PheX-M_at	1058.8	P	0.00006
AFFX-PheX-3_at	1755.5	P	0.000044
AFFX-ThrX-5_at	25.5	A	0.672921
AFFX-ThrX-M_at	6.6	A	0.631562

Total number of rows: 45101

Table truncated, full table size 1206 Kbytes.

Supplementary data files not provided