GEO help: Mouse over screen elements for information.
Status
Public on Sep 26, 2005
Title
Control replicate 3
Sample type
RNA
Source name
Mouse heart
Organism
Mus musculus Characteristics
Control, untreated
Extracted molecule
total RNA
Extraction protocol
Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Control, untreated
Data processing
Robust Multichip Analysis (RMA)
Submission date
Aug 05, 2005
Last update date
Sep 26, 2005
Contact name
Norman Buroker
E-mail(s)
Norman.Buroker@seattlechildrens.org Organization name
Seattle Children's Hospital
Lab
Portman
Street address
4800 Sand Point Way N.E.
City
Seattle
State/province
WA
ZIP/Postal code
98105
Country
USA
Platform ID
GPL1261 Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
360.8
P
0.000754
AFFX-BioB-M_at
515.2
P
0.000195
AFFX-BioB-3_at
316.7
P
0.000195
AFFX-BioC-5_at
916
P
0.000127
AFFX-BioC-3_at
766.6
P
0.000052
AFFX-BioDn-5_at
851.5
P
0.000044
AFFX-BioDn-3_at
4668.5
P
0.00007
AFFX-CreX-5_at
10483.8
P
0.000052
AFFX-CreX-3_at
12354
P
0.000044
AFFX-DapX-5_at
16182
P
0.000044
AFFX-DapX-M_at
16943.1
P
0.000044
AFFX-DapX-3_at
17727.1
P
0.000044
AFFX-LysX-5_at
13.7
A
0.574038
AFFX-LysX-M_at
45.5
A
0.41138
AFFX-LysX-3_at
54
P
0.000754
AFFX-PheX-5_at
2.4
A
0.852061
AFFX-PheX-M_at
5.6
A
0.852061
AFFX-PheX-3_at
11.1
A
0.58862
AFFX-ThrX-5_at
4.3
A
0.963431
AFFX-ThrX-M_at
15.3
A
0.455413
45101 1204 Kbytes .Supplementary data files not provided
