Primary mouse embryonic fibroblasts (MEFs) were generated from e14.5 embryos that contain a deletion in the CH1 domain of both alleles of p300 and one allele of CBP. The remaining allele of CBP was a conditional knock out allele. At passage 3 MEFs were infected with Cre Adenovirus and grown until they had expanded at least 100 fold. Subconfluent MEFs were treated 0.1% oxygen (hypoxia), with 5% carbon dioxide at 37 C in a humid chamber for 6hrs. At the start of treatment, medium was removed and replaced with medium (DMEM+10% FBS+pen-strep+ l-glu) that had been preequilibrated overnight in hypoxia. Immediately after treatment, cells were lysed in Trizol for RNA extraction. Sample Type: Mouse Total RNA Project: Brindle Sample User: jmorris Probe Array Type: Mouse430_2 Barcode: @52002900410450110805400482905872 Probe Array Lot: 4004829 Expiration Date: Nov 08 2005 12:00AM Experiment User: jmorris Algorithm: Statistical HZ:4 VZ:4,BG:4,SmoothFactorBG:100,Epsilon:0.5,BF:,Alpha1:0.05,Alpha2:0.065,Tau:0.015,Gamma1H:0.0025,Gamma1L:0.0025,Gamma2H:0.003,Gamma2L:0.003,Perturbation:1.1,TGT:500,NF:1.000000000000,SF:3.580704689026,SFGene:All Background:Avg:60.57,Stdev:1.88,Max:66.3,Min:55.2 Noise:Avg:3.12,Stdev:0.15,Max:3.6,Min:2.6 RawQ:2.42 Corner+:Avg:210,Count:32 Corner-:Avg:13232,Count:32 Central-:Avg:13446,Count:9
Two transactivation mechanisms are responsible for the bulk of HIF-1alpha-responsive gene expression
Data table header descriptions
ID_REF
As defined by Affymetrix, these are the probe set identifiers, each of which is unique to a specific probe set defining a specific region of a single gene or set.
VALUE
This is the final calculated meauremeent for each probe set identifer that has been made comparable, through scaling or normalization, accross all samples and rows.
DETECTION P-VALUE
Defines the confidence in the calculated signal accurately reflecting the expression level of the specific gene or est that the probe set identifies.
