|
Status |
Public on Feb 07, 2012 |
Title |
patient2-c2-2 |
Sample type |
RNA |
|
|
Source name |
non-pregnant myometrial strips
|
Organism |
Homo sapiens |
Characteristics |
tissue: non-pregnant myometrial strips treatment: C2 patient: P2
|
Treatment protocol |
strips were treated for 24 hours in D-val medium, 10% Fetal Calf Serum at 37oC with either TSA, Compound 2 or vehicle (Control)
|
Growth protocol |
strips were maintained in D-val medium, 10% Fetal Calf Serum at 37oC
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with Ambion RNAqueous kit according to manufacturers instructions
|
Label |
biotin
|
Label protocol |
Illumina TotalPrep-96 RNA amplification kit was used to generate Biotin labeled cRNA according to manufacturers instructions
|
|
|
Hybridization protocol |
750ng of biotinylated cRNA was hybridised to Illumina HT-12 v4 arrays using methods adapted from the Whole-Genome Gene Expression Direct Hybridization Assay Illumina protocol by ServiceXS
|
Scan protocol |
Samples scanned using Illumina iScan array scanner to Illumina protocols
|
Description |
101461-06-2
|
Data processing |
Raw data is the output from Illumina Genomestudio v 2010.1 Normalized data is VST/RSN from lumi BioConductor package v2.8
|
|
|
Submission date |
Feb 07, 2011 |
Last update date |
Feb 07, 2012 |
Contact name |
Simon Cockell |
E-mail(s) |
simon.cockell@newcastle.ac.uk
|
Organization name |
Newcastle University
|
Street address |
Framlington Place
|
City |
Newcastle |
ZIP/Postal code |
NE2 4HH |
Country |
United Kingdom |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE27124 |
Comparison of pan-inhibitor of HDACS (TSA) to specific HDAC8 inhibitor (HDAC Inhibitor XIX, Compound 2) in human myometrium |
|