|
| Status |
Public on Feb 07, 2012 |
| Title |
patient1-tsa1 |
| Sample type |
RNA |
| |
|
| Source name |
non-pregnant myometrial strips
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: non-pregnant myometrial strips
treatment: TSA
patient: P1
|
| Treatment protocol |
strips were treated for 24 hours in D-val medium, 10% Fetal Calf Serum at 37oC with either TSA, Compound 2 or vehicle (Control)
|
| Growth protocol |
strips were maintained in D-val medium, 10% Fetal Calf Serum at 37oC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Ambion RNAqueous kit according to manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
Illumina TotalPrep-96 RNA amplification kit was used to generate Biotin labeled cRNA according to manufacturers instructions
|
| |
|
| Hybridization protocol |
750ng of biotinylated cRNA was hybridised to Illumina HT-12 v4 arrays using methods adapted from the Whole-Genome Gene Expression Direct Hybridization Assay Illumina protocol by ServiceXS
|
| Scan protocol |
Samples scanned using Illumina iScan array scanner to Illumina protocols
|
| Description |
101461-02
|
| Data processing |
Raw data is the output from Illumina Genomestudio v 2010.1
Normalized data is VST/RSN from lumi BioConductor package v2.8
|
| |
|
| Submission date |
Feb 07, 2011 |
| Last update date |
Feb 07, 2012 |
| Contact name |
Simon Cockell |
| E-mail(s) |
simon.cockell@newcastle.ac.uk
|
| Organization name |
Newcastle University
|
| Street address |
Framlington Place
|
| City |
Newcastle |
| ZIP/Postal code |
NE2 4HH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE27124 |
Comparison of pan-inhibitor of HDACS (TSA) to specific HDAC8 inhibitor (HDAC Inhibitor XIX, Compound 2) in human myometrium |
|
