|
| Status |
Public on Jul 12, 2006 |
| Title |
CD8+H7aTet+_04 |
| Sample type |
RNA |
| |
|
| Source name |
Spleen Tcells day 14
|
| Organism |
Mus musculus |
| Characteristics |
Strain: B10.C-H7b(47N)/SN
Gender: Female
Age: 6-8wks
Tissue:Spleen
|
| Biomaterial provider |
Dr. Claude Perreault
|
| Treatment protocol |
Immunization of a female B10.C-H7b(47N)/SN with splenocytes from male B10.C-H7b(47N)/SN + male C57BL/10Snj
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from sorted populations using TRIzol (Invitrogen/Life Technologies)
|
| Label |
biotin + R-phycoerythrin steptavidin
|
| Label protocol |
Labeling of samples was performed according to standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
EukGE-WS2v5_450
|
| Scan protocol |
Pixel Size 1.56
Filter 570
Scan Temperature
Scan Date Aug 30 2004 05:59PM
Scanner ID 50207280
Number of Scans 1
Scanner Type M10
|
| Description |
B10.C-H7b(47N)/SN female mice were immunized by i.p. co-injection of C57BL/10Snj male and B10.C-H7b(47N)/SN male spleen cells. Fourteen days later, single-cell suspensions were prepared from the spleen of immunized mice. CD8+H7aTet+, CD8+HYTet+ and CD8+Tet- cells were sorted using a FACSVantage SE with Diva option (BD Biosciences, San Jose). Purity of sorted cell populations was always ≥ 98%. Tet+ populations containing between 8-300 thousand cells and Tet- population containing 2-3 million cells were obtained for each mouse and processed separately.
|
| Data processing |
MAS5
|
| |
|
| Submission date |
Jun 30, 2005 |
| Last update date |
Sep 08, 2006 |
| Contact name |
Etienne Caron |
| Organization name |
Hopital Maisonneuve Rosemont
|
| Department |
Recherche
|
| Lab |
Dr Claude Perreault
|
| Street address |
5415 Boul. L'Assomption
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H1T 2M4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE2924 |
Immunodomination leads to selective expansion of the fittest CD8 T cells |
|
