|
| Status |
Public on Jan 06, 2011 |
| Title |
92.1 - control #1 - replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
92.1 cells
|
| Organism |
Homo sapiens |
| Characteristics |
knockdown: Control
cell line: 92.1
|
| Treatment protocol |
All lines transfected using HiPerFect (Qiagen) system according to manufacturer's instructions. Silencing constructs consisted of either control #1 siRNA (Ambion) or Silencer Select BAP1. 5 days post transfection cells were harvested for RNA extraction.
|
| Growth protocol |
Grown in RPMI-1640 (Lonza) supplemented with 10% fetal bovine serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNEasy RNA isolation kit according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Illumina TotalPrep RNA Amplification kit (Ambion, AMIL1791). Cleanup with DNA Clean-up and Concentrator 5 columns (zymo research).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2 - separate transfection
|
| Data processing |
Data were analyzed using beadarray and limma R packages. Raw data were log2 transformed and quantile normalized. siBAP1 versus siControl contrast was analyzed against the fitted linear model using eBayes functions in limma.
|
| |
|
| Submission date |
Oct 22, 2010 |
| Last update date |
Jan 06, 2011 |
| Contact name |
Elisha D.O. Roberson |
| E-mail(s) |
eroberso@dom.wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Department |
Internal Medicine & Genetics
|
| Lab |
Roberson Lab
|
| Street address |
660 South Euclid Ave., CB 8045
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE24896 |
Global expression analysis of BAP1 knockdown in transfected 92.1 cells |
|
