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Sample GSM60420 Query DataSets for GSM60420
Status Public on Dec 06, 2005
Title tumor immune evasion - P3 cell line-replicate 3
Sample type RNA
 
Source name mouse cancer cell line, P3
Organism Mus musculus
Characteristics strain - C57BL/6
gender - female
tissue - P0/TC-1 is a cancer cell line derived from lung fibroblasts. P3 is derived from P0.
Treatment protocol P3 is derived from P0 through 3 rounds of in vivo immune selection. P3 is a highly immune resistant cell line.
Growth protocol The cell line was cultured in vitro in 10-cm dishes until 70-75% confluency and then total RNA was isolated.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent (Invitrogen), followed by a clean-up step using RNeasy mini kit (Qiagen).
Label biotin-labeled cRNA, detected with streptavidin phycoerythin conjugate
 
Description This is one of 4 biological replicates for P3 cell line.
Data processing background correction made with RMA, Quantile Normalization, GC-RMA
 
Submission date Jun 06, 2005
Last update date Jun 07, 2005
Contact name Ken-Yu Lin
E-mail(s) kenyulin@jhmi.edu
Phone 410-614-4906
Organization name Johns Hopkins University School of Medicine
Department Pathology
Lab T.-C. Wu
Street address 720 Rutland Ave, Ross 512
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platform ID GPL1261
Series (1)
GSE2774 Identification of tumor immune evasion mechanism using P0 and P3 cell lines

Data table header descriptions
ID_REF
VALUE data with quantile normalization

Data table
ID_REF VALUE
1415670_at 2839.754723
1415671_at 627.081278
1415672_at 2941.135791
1415673_at 683.616667
1415674_a_at 579.113231
1415675_at 524.01914
1415676_a_at 7215.163041
1415677_at 949.493207
1415678_at 1179.016758
1415679_at 1612.718075
1415680_at 2696.087056
1415681_at 646.243772
1415682_at 1003.695596
1415683_at 2181.549761
1415684_at 351.697852
1415685_at 296.609998
1415686_at 1377.806798
1415687_a_at 3948.064997
1415688_at 976.064743
1415689_s_at 97.126637

Total number of rows: 45101

Table truncated, full table size 932 Kbytes.




Supplementary data files not provided

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