|
| Status |
Public on Nov 01, 2021 |
| Title |
Nacc in monkey P, replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
Nacc in monkey P
|
| Organism |
Macaca mulatta |
| Characteristics |
age: 5 years
genotype: wild type
tissue: Nacc
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We isolated nuclei using a loose glass dounce homogenizer followed by a tight glass homogenizer in EZ PREP buffer (Millipore Sigma, Cat# NUC-101).
Single nuclei RNA libraries were prepared for sequencing using standard 10x Chromium Single Cell 3’ Reagent kits v3 Chemistry (10x Genomics, Cat# PN-1000075).
10x Chromium Single Cell 3' v3 chemistry.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
mRNA for single nuclei
WS_5N_STA706A11_S5
|
| Data processing |
Basecalls performed using RTA3
10x cellranger count was used to align reads to a custom reference genome
Data were filtered, normalized, integrated, clutered and visualized using a Seurat v3 pipline
Supplementary_files_format_and_content: rds files containing filtered counting matrix or raw counting matrix
Supplementary_files_format_and_content: rds files containing clustering information for major cell classes
Supplementary_files_format_and_content: rds files containing clustering information for MSN types
|
| |
|
| Submission date |
Feb 28, 2021 |
| Last update date |
Nov 01, 2021 |
| Contact name |
William R. Stauffer |
| E-mail(s) |
wrs@pitt.edu
|
| Phone |
4124008246
|
| Organization name |
University of Pittsburgh
|
| Department |
Neurobiology
|
| Street address |
3501 Fifth Ave
|
| City |
PITTSBURGH |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL27943 |
| Series (1) |
| GSE167920 |
Transcriptional and anatomical diversity of medium spiny neurons in the primate striatum. |
|
| Relations |
| BioSample |
SAMN18090506 |
| SRA |
SRX10191360 |
