|
Status |
Public on Nov 01, 2021 |
Title |
Caudate in monkey P, replicate 2 |
Sample type |
SRA |
|
|
Source name |
Caudate in monkey P
|
Organism |
Macaca mulatta |
Characteristics |
age: 5 years genotype: wild type tissue: Caudate
|
Extracted molecule |
total RNA |
Extraction protocol |
We isolated nuclei using a loose glass dounce homogenizer followed by a tight glass homogenizer in EZ PREP buffer (Millipore Sigma, Cat# NUC-101). Single nuclei RNA libraries were prepared for sequencing using standard 10x Chromium Single Cell 3’ Reagent kits v3 Chemistry (10x Genomics, Cat# PN-1000075). 10x Chromium Single Cell 3' v3 chemistry.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
mRNA for single nuclei WS_4N_STA706A10_S4
|
Data processing |
Basecalls performed using RTA3 10x cellranger count was used to align reads to a custom reference genome Data were filtered, normalized, integrated, clutered and visualized using a Seurat v3 pipline Supplementary_files_format_and_content: rds files containing filtered counting matrix or raw counting matrix Supplementary_files_format_and_content: rds files containing clustering information for major cell classes Supplementary_files_format_and_content: rds files containing clustering information for MSN types
|
|
|
Submission date |
Feb 28, 2021 |
Last update date |
Nov 01, 2021 |
Contact name |
William R. Stauffer |
E-mail(s) |
wrs@pitt.edu
|
Phone |
4124008246
|
Organization name |
University of Pittsburgh
|
Department |
Neurobiology
|
Street address |
3501 Fifth Ave
|
City |
PITTSBURGH |
State/province |
PA |
ZIP/Postal code |
15213 |
Country |
USA |
|
|
Platform ID |
GPL27943 |
Series (1) |
GSE167920 |
Transcriptional and anatomical diversity of medium spiny neurons in the primate striatum. |
|
Relations |
BioSample |
SAMN18090508 |
SRA |
SRX10191358 |