|
| Status |
Public on Nov 25, 2009 |
| Title |
25 ng/hr leptin_7 |
| Sample type |
RNA |
| |
|
| Source name |
adult male liver, treated with 25 ng/hr leptin
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
agent: untreated
dose: 25 ng/hr
time: 12 days
|
| Treatment protocol |
Animals were treated for 12 days with leptin at defined doses.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Livers were extracted and flash frozen and later processed for RNA using a Trizol protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
adult male mouse
4240351001_G
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Nov 25, 2009 |
| Last update date |
Mar 08, 2017 |
| Contact name |
Kristina Hedbacker |
| E-mail(s) |
kristina.hedbaker@rockefeller.edu
|
| Organization name |
Rockefeller University
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE19185 |
Low dose Leptin (25 ng/hr and 12.5 ng/hr) in ob/ob mice |
|
