|
| Status |
Public on Sep 23, 2009 |
| Title |
KO_RSV 1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse/ Lung, PKR deficient, RSV treated
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
strain: PKR-/-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol/Qiagen RNA clean up
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
24 hr infection
|
| Data processing |
Data were obtained and processed with the Agilent Feature Extraction Software (v9.5). The resulting data were imported into Rosetta Resolver (v7.1) and log2 intensities were exported.
|
| |
|
| Submission date |
Sep 18, 2009 |
| Last update date |
Sep 22, 2009 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE18170 |
Double-Stranded RNA-Activated Protein Kinase Regulates early innate immune responses during RSV Infection |
|
