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Sample GSM409665 Query DataSets for GSM409665
Status Public on Mar 18, 2010
Title MM - t(11;14) - p1077
Sample type RNA
 
Source name Bone marrow, MM
Organism Homo sapiens
Characteristics tissue: bone marrow
cell type: CD138-positive plasma cell
disease: multiple myeloma
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from normal and tumor plasma cells using miRNEasy Mini Kit (Qiagen, Valencia, USA) following manufacturer's protocol. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA).
Label biotin
Label protocol Labelling and hybridizations were performed according to protocols from Affymetrix. Briefly, 100-300 ng of total RNA were amplified and labeled using the WT Sense Target labelling and control reagents kit (Affymetrix Inc., Santa Clara, CA, USA)
 
Hybridization protocol Labeled RNA was hybridized to Human Gene 1.0 ST Array
Scan protocol Washing and scanning were performed using GeneChip System of Affymetrix (GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450 and GeneChip Scanner 7G).
Description MM - t(11;14) - p1077
Data processing Expression value for each probe set was calculated using RMAExpress program that uses RMA (Robust Multi-Array Average) algorithm.
 
Submission date May 28, 2009
Last update date Mar 17, 2010
Contact name Norma Carmen Gutierrez
E-mail(s) normagu@usal.es
Phone 34923291375
Organization name University Hospital
Department Hematology
Street address
City Salamanca
ZIP/Postal code 37007
Country Spain
 
Platform ID GPL6244
Series (1)
GSE16558 MicroRNAs in Myeloma

Data table header descriptions
ID_REF
VALUE RMA-normalized expression value

Data table
ID_REF VALUE
7896736 4.404415
7896738 2.545751
7896740 3.22033
7896742 5.234983
7896744 3.505056
7896746 8.942639
7896748 6.372976
7896750 2.545892
7896752 8.800142
7896754 3.843753
7896756 4.006554
7896759 5.724685
7896761 6.181169
7896779 6.268857
7896798 6.452456
7896817 6.308562
7896822 5.320984
7896859 4.740277
7896861 3.370751
7896863 5.794893

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM409665.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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