|
| Status |
Public on Mar 18, 2010 |
| Title |
MM - RB deletion and P53 deletion - p895 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow, MM
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bone marrow
cell type: CD138-positive plasma cell
disease: multiple myeloma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from normal and tumor plasma cells using miRNEasy Mini Kit (Qiagen, Valencia, USA) following manufacturer's protocol. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA).
|
| Label |
biotin
|
| Label protocol |
Labelling and hybridizations were performed according to protocols from Affymetrix. Briefly, 100-300 ng of total RNA were amplified and labeled using the WT Sense Target labelling and control reagents kit (Affymetrix Inc., Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to Human Gene 1.0 ST Array
|
| Scan protocol |
Washing and scanning were performed using GeneChip System of Affymetrix (GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450 and GeneChip Scanner 7G).
|
| Description |
MM - RB deletion and P53 deletion - p895
|
| Data processing |
Expression value for each probe set was calculated using RMAExpress program that uses RMA (Robust Multi-Array Average) algorithm.
|
| |
|
| Submission date |
May 28, 2009 |
| Last update date |
Mar 17, 2010 |
| Contact name |
Norma Carmen Gutierrez |
| E-mail(s) |
normagu@usal.es
|
| Phone |
34923291375
|
| Organization name |
University Hospital
|
| Department |
Hematology
|
| Street address |
|
| City |
Salamanca |
| ZIP/Postal code |
37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
|
