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Sample GSM404003 Query DataSets for GSM404003
Status Public on May 16, 2009
Title CD4TESTCJ#2
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
genetic background: 4+ 8- B220-
phenotype markers: 4+8-B220-
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description CD4 Test CJ#2
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date May 15, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 18.7015
10344622 298.8198
10344624 513.3145
10344633 806.2379
10344637 305.3504
10344653 16.1458
10344658 531.2543
10344674 8.3545
10344679 34.2302
10344707 410.6231
10344713 366.7385
10344723 211.5094
10344725 47.866
10344741 3259.5043
10344743 25.6944
10344750 605.5778
10344772 34.4139
10344789 263.7138
10344797 180.0189
10344799 267.2351

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM404003_EA07068_82674_MoGene_CD4TESTCJ#2.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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