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Sample GSM404001 Query DataSets for GSM404001
Status Public on May 16, 2009
Title CD4TESTJS#2
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics age: 6 weeks
genetic background: 4+ 8- B220-
phenotype markers: 4+8-B220-
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description CD4 Test JS#2
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date May 15, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 16.8082
10344622 253.0841
10344624 364.7932
10344633 628.205
10344637 180.254
10344653 17.1187
10344658 221.0474
10344674 12.0842
10344679 55.167
10344707 280.5771
10344713 375.5592
10344723 188.2992
10344725 54.4878
10344741 2093.4154
10344743 27.0825
10344750 391.9319
10344772 24.6407
10344789 117.0308
10344797 283.641
10344799 140.9415

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM404001_EA07068_82677_MoGene_CD4TESTJS#2.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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