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Sample GSM399376 Query DataSets for GSM399376
Status Public on May 02, 2009
Title T.4SP69+.Th#1
Sample type RNA
 
Source name thymus
Organism Mus musculus
Characteristics age: 6 weeks
exclusion markers: 11b- 11c- 19- 49b- Gr1- TER119-
genetic background: C57BL/6J
phenotype markers: 4+ 8- TCRhi 69+
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Glass slide teasing Flow sorting
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description T.4SP69+.Th#1, CD4 Single-Positive, Intermediate
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Apr 30, 2009
Last update date May 24, 2016
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE73864

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 15.6633
10344622 316.8762
10344624 512.9953
10344633 773.1536
10344637 256.8905
10344653 13.0213
10344658 273.7661
10344674 8.3859
10344679 35.96
10344707 221.9814
10344713 453.3355
10344723 246.2772
10344725 45.6769
10344741 3457.9083
10344743 26.9647
10344750 622.349
10344772 27.8173
10344789 205.3766
10344797 268.0561
10344799 178.5682

Total number of rows: 24922

Table truncated, full table size 422 Kbytes.




Supplementary file Size Download File type/resource
GSM399376_EA07068_52768_MoGene_T.4SP69+.Th_#1.cel.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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