|
| Status |
Public on Feb 07, 2009 |
| Title |
retina_time 0_CD133-_donorA |
| Sample type |
RNA |
| |
|
| Source name |
CD133-
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: retina
|
| Treatment protocol |
Retina was dissociated to form a single cell population and CD133+ & CD133- cells were isolated using magnetic beads
|
| Growth protocol |
human retina was disected from post mortem eyes (with research consent)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
300ng of RNA were isolated from magnetically sorted cells using RNeasy mini® kit according to the manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
GeneChip Whole Transcript (WT) sense target labeling assay manual, version 4 - starting with 300ng RNA and following the Total RNA labeling protocol to prepare targets for GeneChip gene 1.0 ST Arrays(omitting RiboMinus procedure).
|
| |
|
| Hybridization protocol |
using the genechip hybridisation, wash and stain kit
|
| Scan protocol |
Array washing and staining as described in chp 6 of above manual using the GeneChip Fluidics station 450 (fluidics script FS450-007). Arrays were scanned on a GeneChip scanner 3000 7G using default scanner settings. Affymetrix GeneChip® Command Console® (AGCC) was used to process all Affymetrix arrays through fluidics and scanning.
|
| Description |
DC_A2_(HuGene-1_0-st-v1).CEL
|
| Data processing |
Gene level probe set summarisation (signal value), calculation of QC metrics and CHP file generation were performed using GeneChip® Expression Console software using the Robust Multichip Analysis (RMA) algorithm
|
| |
|
| Submission date |
Feb 06, 2009 |
| Last update date |
Feb 06, 2009 |
| Contact name |
DEBRA ANN CARTER |
| E-mail(s) |
debbie.carter@bristol.ac.uk
|
| Organization name |
BRISTOL UNIVERSITY
|
| Department |
CLINICAL SCIENCE @ SOUTH BRISTOL
|
| Lab |
OPHTHALMOLOGY
|
| Street address |
BRISTOL EYE HOSPITAL, LOWER MAUDLIN STREET
|
| City |
BRISTOL |
| ZIP/Postal code |
BS1 2LX |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE14733 |
Understanding adult human progenitor cells |
|
