|
| Status |
Public on Apr 01, 2013 |
| Title |
MuRF1 -/- // MuRF2 +/-: Biological replicate 3. |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Total RNA from murine heart labeled with Cyanine-5 (red).
|
| Organism |
Mus musculus |
| Characteristics |
Cardiac tissue
untreated
|
| Treatment protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Qiagen All-Prep kit following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
50 ng of total RNA were amplified and labeled with Cy3-CTP using the Agilent Low Input RNA amplification kit exactly as described by the manufacturer.
|
| |
|
| Channel 2 |
| Source name |
Total RNA from pooled whole mouse neonates (day 1), labeled with Cyanine-3 (green).
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Age: postnatal Day 1
Tissue: whole pup
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Qiagen All-Prep kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
50 ng of total RNA were amplified and labeled with Cy3-CTP using the Agilent Low Input RNA amplification kit exactly as described by the manufacturer.
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent In Situ Hybridization Kit Plus) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Axon Instruments GenePix 4000b scanner.
Images were quantified using Genepix Pro (version 6.0).
|
| Description |
MuRF1 -/- // MuRF2 +/-: Biological replicate 3.
|
| Data processing |
LOWESS normalized, background subtracted data obtained from processed Red signal/processed Green signal by Genepix Pro V 6.0. Data was subsequently downloaded and filtered for features present in less than 70% of arrays.
|
| |
|
| Submission date |
Jan 22, 2009 |
| Last update date |
Apr 01, 2013 |
| Contact name |
Monte Shaw Willis |
| E-mail(s) |
monte_willis@med.unc.edu
|
| Phone |
919-843-1938
|
| Organization name |
University of North Carolina
|
| Department |
Pathology & Laboratory Medicine
|
| Street address |
103 Mason Farm Road, MBRB 2340B
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599-7525 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE14512 |
Muscle Ring Finger 1 (MuRF1) and MuRF2 are Necessary but Functionally Redundant During Developmental Cardiac Growth and Regulate E2F1-Mediated Gene Expression In Vivo |
|
