|
| Status |
Public on Oct 08, 2008 |
| Title |
control retina T48-sample3 |
| Sample type |
RNA |
| |
|
| Source name |
lesioned retina 48hrs after light onset
|
| Organism |
Danio rerio |
| Characteristics |
wild type, adult Danio retina
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pico Pure isolation kit (Arcturus, Molecular Devices Corporation)
|
| Label |
biotin
|
| Label protocol |
mRNA was amplified from total RNA using the RiboAmp HS RNA amplification kit (Arcturus, Molecular Devices Corporation.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5ug of cRNA were hybridized on GeneChip Zebrafish Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene Expression from lesioned retina 48hrs after light onset.
|
| Data processing |
The data were analyzed with BRB Array Tools 3.6 using default analysis settings and global scaling for RMA normalization.
|
| |
|
| Submission date |
Oct 06, 2008 |
| Last update date |
Oct 07, 2008 |
| Contact name |
Peter F. Hitchcock |
| E-mail(s) |
peterh@umich.edu
|
| Phone |
734-763-8169
|
| Fax |
734-936-7231
|
| Organization name |
University of Michigan
|
| Department |
Ophthy and Vis Sci
|
| Street address |
1000 Wall Street
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE13068 |
Identification of the molecular signatures integral to regenerating photoreceptors in the retina of the zebrafish |
|
