|
| Status |
Public on Sep 18, 2008 |
| Title |
Zebrafish_wt_24hpf, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Zebrafish embryo harvested at 24hpf
|
| Organism |
Danio rerio |
| Characteristics |
Zebrafish wild-type whole-embryo RNA
|
| Treatment protocol |
Equal number of embryos (WT siblings vs. fogsk8 mutants) at 24 hpf was dechorionated manually or by Pronase K treatment and Trizol (Invitrogen) used to extract total RNA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
According to manufacturer's (Affymetrix) recommendations
|
| |
|
| Hybridization protocol |
According to manufacturer's (Affymetrix) recommendations
|
| Scan protocol |
According to manufacturer's (Affymetrix) recommendations
|
| Description |
Gene expression data from wild-type embryos at 24 hours post fertilization
|
| Data processing |
Gene intensity values for the Affymetrix Zebrafish Genome Array CEL files were obtained using the GC-RMA package from Bioconductor.
|
| |
|
| Submission date |
Sep 17, 2008 |
| Last update date |
Sep 17, 2008 |
| Contact name |
Nathan Salomonis |
| E-mail(s) |
nathan.salomonis@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital
|
| Department |
Biomedical Informatics
|
| Lab |
Nathan Salomonis
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE12826 |
Zebrafish 24hpf mutant sk8 gene expression |
|
